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Literature summary extracted from

  • Jung, J.H.; Lee, S.B.
    Identification and characterization of Thermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases (2005), Biotechnol. Bioprocess Eng., 10, 535-539.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.45 dephosphorylation with potato acid phosphatase or bacterial alkaline phosphatase leads to complete loss of activity Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.45 34000
-
8 * 34000, denaturing SDS-PAGE Thermoplasma acidophilum
2.7.1.45 36390
-
calculated from amino acid sequence Thermoplasma acidophilum
2.7.1.45 260000
-
gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.45 Thermoplasma acidophilum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.45 phosphoprotein dephosphorylation with potato acid phosphatase or bacterial alkaline phosphatase, leads to complete loss of activity Thermoplasma acidophilum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.45 DEAE Sepharose column chromatography, Q Sepharose column chromatography, Affi-gel blue column chromatography, Sephadex G-75 gel filtration, and Mono Q HR 5/5 column chromatography Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.45 additional information
-
15.6 units/mg, at 50°C in 50 mM Tris-HCl, pH 8.0 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.45 ATP + 2-dehydro-3-deoxy-D-gluconate
-
Thermoplasma acidophilum ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
?
2.7.1.45 additional information D-gluconate, 2-keto-D-gluconate, 5-keto-D-gluconate, N-acetylglucosamine, and D-xylose are no substrates Thermoplasma acidophilum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.45 homooctamer 8 * 34000, denaturing SDS-PAGE Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.45 2-keto-3-deoxy-D-gluconate kinase
-
Thermoplasma acidophilum
2.7.1.45 KDG kinase
-
Thermoplasma acidophilum
2.7.1.45 Ta0122
-
Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.45 70
-
-
Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.45 4 5
-
Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.45 ATP
-
Thermoplasma acidophilum