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Literature summary extracted from

  • Ingram, C.U.; Bommer, M.; Smith, M.E.; Dalby, P.A.; Ward, J.M.; Hailes, H.C.; Lye, G.J.
    One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine:pyruvate transaminase pathway in Escherichia coli (2006), Biotechnol. Bioeng., 96, 559-569.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.2 expressed in Escherichia coli strain BL21gold(DE3) Pseudomonas aeruginosa
2.6.1.18 expressed in Escherichia coli BL21gold(DE3) cells Pseudomonas aeruginosa

General Stability

EC Number General Stability Organism
2.6.1.18 the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.18 43000
-
 4 * 43000 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate Pseudomonas aeruginosa
-
 pyruvate + L-glutamate
-
 ?
2.6.1.18 beta-alanine + pyruvate Pseudomonas aeruginosa
-
 malonic semialdehyde + L-alanine
-
 ?
2.6.1.18 beta-alanine + pyruvate Pseudomonas aeruginosa PAO2
-
 malonic semialdehyde + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Pseudomonas aeruginosa
-
-
-
2.6.1.2 Pseudomonas aeruginosa PAO2
-
-
-
2.6.1.18 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.18 nickel column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Pseudomonas aeruginosa pyruvate + L-glutamate
-
 ?
2.6.1.2 L-erythrulose + ?
-
 Pseudomonas aeruginosa 2-amino-1,3,4-butanetriol + ?
-
 ?
2.6.1.2 L-methylbenzylamine + 2-oxoglutarate
-
 Pseudomonas aeruginosa ?
-
 ?
2.6.1.2 additional information phenylserine, phenylaminopropanol, phenylaminopropandiol, ethylbenzylamine, and phenylglycinol are not accepted as substrates Pseudomonas aeruginosa ?
-
 ?
2.6.1.2 additional information phenylserine, phenylaminopropanol, phenylaminopropandiol, ethylbenzylamine, and phenylglycinol are not accepted as substrates Pseudomonas aeruginosa PAO2 ?
-
 ?
2.6.1.18 (S)-alpha-methylbenzylamine + pyruvate
-
 Pseudomonas aeruginosa acetophenone + L-alanine
-
 ?
2.6.1.18 (S)-alpha-methylbenzylamine + pyruvate
-
 Pseudomonas aeruginosa PAO2 acetophenone + L-alanine
-
 ?
2.6.1.18 beta-alanine + pyruvate
-
 Pseudomonas aeruginosa malonic semialdehyde + L-alanine
-
 ?
2.6.1.18 beta-alanine + pyruvate
-
 Pseudomonas aeruginosa PAO2 malonic semialdehyde + L-alanine
-
 ?
2.6.1.18 L-erythrulose + pyruvate
-
 Pseudomonas aeruginosa 2-amino-1,3,4-butanetriol + ?
-
 ?
2.6.1.18 L-erythrulose + pyruvate
-
 Pseudomonas aeruginosa PAO2 2-amino-1,3,4-butanetriol + ?
-
 ?
2.6.1.18 additional information D-methylbenzylamine is not accepted as an amine donor Pseudomonas aeruginosa ?
-
 ?
2.6.1.18 additional information D-methylbenzylamine is not accepted as an amine donor Pseudomonas aeruginosa PAO2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.18 tetramer 4 * 43000 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 beta-A:P TAm
-
 Pseudomonas aeruginosa
2.6.1.2 beta-alanine: pyruvate transaminase
-
 Pseudomonas aeruginosa
2.6.1.18 beta-A:P TAm
-
 Pseudomonas aeruginosa
2.6.1.18 beta-alanine:pyruvate transaminase
-
 Pseudomonas aeruginosa
2.6.1.18 PAO2
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.18 25
-
 the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 7
-
-
 Pseudomonas aeruginosa
2.6.1.18 7
-
 for reactions with (S)-alpha-methylbenzylamine and L-erythrulose Pseudomonas aeruginosa
2.6.1.18 8.5
-
 above pH 8.5, for reactions with the natural substrates beta-alanine and pyruvate Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate 2 mM, required Pseudomonas aeruginosa
2.6.1.18 pyridoxal 5'-phosphate
-
 Pseudomonas aeruginosa