EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.25 | phylogenetic analysis | Methylosinus trichosporium |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.25 | instability of the enzyme during purification is probably due to loss of iron | Methylosinus trichosporium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | Dithionite | 34% inhibition at 5 mM | Methylosinus trichosporium | |
1.14.13.25 | DTT | 24% inhibition at 20 mM | Methylosinus trichosporium | |
1.14.13.25 | glycerol | 40% inhibition at 15% | Methylosinus trichosporium | |
1.14.13.25 | N2 | N2 atmosphere causes 28% inhibition | Methylosinus trichosporium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | Iron | non-heme iron, 3.02 mol iron per mol of enzyme, addition of exogenous FeCl2 or FeCl3 does not affect the enzyme activity | Methylosinus trichosporium | |
1.14.13.25 | additional information | the enzyme contains very low or no amounts of copper and zinc | Methylosinus trichosporium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 23000 | - |
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE | Methylosinus trichosporium |
1.14.13.25 | 36000 | - |
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE | Methylosinus trichosporium |
1.14.13.25 | 58000 | - |
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE | Methylosinus trichosporium |
1.14.13.25 | 201300 | - |
gel filtration | Methylosinus trichosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NAD(P)H + O2 | Methylosinus trichosporium | - |
methanol + NAD(P)+ + H2O | - |
? | |
1.14.13.25 | methane + NAD(P)H + O2 | Methylosinus trichosporium IMV 3011 | - |
methanol + NAD(P)+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
1.14.13.25 | Methylosinus trichosporium IMV 3011 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.25 | native enzyme 8.08fold by anion exchange chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography, to 95% purity | Methylosinus trichosporium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 0.604 | - |
purified enzyme, substrate propylene | Methylosinus trichosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylosinus trichosporium IMV 3011 | methanol + NAD(P)+ + H2O | - |
? | |
1.14.13.25 | propylene + NADH + O2 | - |
Methylosinus trichosporium | propylene epoxide + NAD+ + H2O | - |
? | |
1.14.13.25 | propylene + NADH + O2 | - |
Methylosinus trichosporium IMV 3011 | propylene epoxide + NAD+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.25 | hexamer | 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE | Methylosinus trichosporium |
1.14.13.25 | More | the enzyme mainly exists of alpha-helical regions, circular dichroism measurement | Methylosinus trichosporium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.25 | methane monooxygenase hydroxylase | - |
Methylosinus trichosporium |
1.14.13.25 | sMMO | - |
Methylosinus trichosporium |
1.14.13.25 | soluble methane monooxygenase hydroxylase | - |
Methylosinus trichosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.25 | 7 | - |
assay at | Methylosinus trichosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | NAD(P)H | - |
Methylosinus trichosporium |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.14.13.25 | Methylosinus trichosporium | isoelectric focusing | - |
5.2 |