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Literature summary extracted from

  • Shaofeng, H.; Shuben, L.; Jiayin, X.; Jianzhong, N.; Chungu, X.; Haidong, T.; Wei, T.
    Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011 (2007), Biosci. Biotechnol. Biochem., 71, 122-129.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 phylogenetic analysis Methylosinus trichosporium

General Stability

EC Number General Stability Organism
1.14.13.25 instability of the enzyme during purification is probably due to loss of iron Methylosinus trichosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.25 Dithionite 34% inhibition at 5 mM Methylosinus trichosporium
1.14.13.25 DTT 24% inhibition at 20 mM Methylosinus trichosporium
1.14.13.25 glycerol 40% inhibition at 15% Methylosinus trichosporium
1.14.13.25 N2 N2 atmosphere causes 28% inhibition Methylosinus trichosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Iron non-heme iron, 3.02 mol iron per mol of enzyme, addition of exogenous FeCl2 or FeCl3 does not affect the enzyme activity Methylosinus trichosporium
1.14.13.25 additional information the enzyme contains very low or no amounts of copper and zinc Methylosinus trichosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 23000
-
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
1.14.13.25 36000
-
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
1.14.13.25 58000
-
2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
1.14.13.25 201300
-
gel filtration Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2 Methylosinus trichosporium
-
methanol + NAD(P)+ + H2O
-
?
1.14.13.25 methane + NAD(P)H + O2 Methylosinus trichosporium IMV 3011
-
methanol + NAD(P)+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium
-
-
-
1.14.13.25 Methylosinus trichosporium IMV 3011
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 native enzyme 8.08fold by anion exchange chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography, to 95% purity Methylosinus trichosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 0.604
-
purified enzyme, substrate propylene Methylosinus trichosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
?
1.14.13.25 methane + NAD(P)H + O2
-
Methylosinus trichosporium IMV 3011 methanol + NAD(P)+ + H2O
-
?
1.14.13.25 propylene + NADH + O2
-
Methylosinus trichosporium propylene epoxide + NAD+ + H2O
-
?
1.14.13.25 propylene + NADH + O2
-
Methylosinus trichosporium IMV 3011 propylene epoxide + NAD+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.25 hexamer 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
1.14.13.25 More the enzyme mainly exists of alpha-helical regions, circular dichroism measurement Methylosinus trichosporium

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 methane monooxygenase hydroxylase
-
Methylosinus trichosporium
1.14.13.25 sMMO
-
Methylosinus trichosporium
1.14.13.25 soluble methane monooxygenase hydroxylase
-
Methylosinus trichosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.25 7
-
assay at Methylosinus trichosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NAD(P)H
-
Methylosinus trichosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.25 Methylosinus trichosporium isoelectric focusing
-
5.2