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Literature summary extracted from

  • Kim, S.; Lee, S.B.
    Characterization of Sulfolobus solfataricus 2-keto-3-deoxy-D-gluconate kinase in the modified Entner-Doudoroff pathway (2006), Biosci. Biotechnol. Biochem., 70, 1308-1316.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.178 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.178 0.14
-
 2-dehydro-3-deoxy-D-gluconate pH 7.2, 60°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.178 34874
-
 2 * 34874, calculated from sequence Saccharolobus solfataricus
2.7.1.178 43000
-
 2 * 43000, SDS-PAGE Saccharolobus solfataricus
2.7.1.178 72000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the Entner–Doudoroff pathway ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus P2 the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the Entner–Doudoroff pathway ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.178 Saccharolobus solfataricus Q97U29
-
-
2.7.1.178 Saccharolobus solfataricus P2 Q97U29
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.178
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the Entner–Doudoroff pathway Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus P2 ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the Entner–Doudoroff pathway Saccharolobus solfataricus P2 ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 additional information no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate Saccharolobus solfataricus ?
-
 ?
2.7.1.178 additional information no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate Saccharolobus solfataricus P2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.178 homodimer 2 * 43000, SDS-PAGE Saccharolobus solfataricus
2.7.1.178 homodimer 2 * 34874, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.178 KDGK
-
 Saccharolobus solfataricus
2.7.1.178 SSO3195
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.178 60
-
 assay at Saccharolobus solfataricus
2.7.1.178 70 80
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.178 80
-
 deactivated above Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.178 60.8
-
 2-dehydro-3-deoxy-D-gluconate pH 7.2, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.178 7 8
-
 Saccharolobus solfataricus
2.7.1.178 7.2
-
 assay at Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.178 Saccharolobus solfataricus calculated from sequence
-
 6.1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.178 434
-
 2-dehydro-3-deoxy-D-gluconate pH 7.2, 60°C Saccharolobus solfataricus