EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
7.1.2.2 | N,N'-dicyclohexylcarbodiimide | 0.1 mM increases ATPase activity by 20% | Acidithiobacillus ferrooxidans | |
7.1.2.2 | sulfite | 50 mM stimulates 4fold | Acidithiobacillus ferrooxidans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
7.1.2.2 | strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system | Acidithiobacillus ferrooxidans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.1.2.2 | azide | 2 mM inhibits 91% ATPase activity | Acidithiobacillus ferrooxidans | |
7.1.2.2 | Cl- | - |
Acidithiobacillus ferrooxidans | |
7.1.2.2 | Cu2+ | 1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
7.1.2.2 | Mn2+ | 1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
7.1.2.2 | additional information | relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
7.1.2.2 | NaCl | inhibits ATPase activity | Acidithiobacillus ferrooxidans | |
7.1.2.2 | Ni2+ | 1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans | |
7.1.2.2 | sulfate | slightly inhibits | Acidithiobacillus ferrooxidans | |
7.1.2.2 | Zn2+ | 1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4 | Acidithiobacillus ferrooxidans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.1.2.2 | 1.8 | - |
ATP | estimated at pH 8.5 at 40°C | Acidithiobacillus ferrooxidans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.1.2.2 | membrane | - |
Acidithiobacillus ferrooxidans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.1.2.2 | Mg2+ | - |
Acidithiobacillus ferrooxidans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.2.2 | Acidithiobacillus ferrooxidans | - |
strain NASF-1, strain ATCC33020 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.1.2.2 | 51fold by chromatographies, purified ATPase does not contain the F0-portion | Acidithiobacillus ferrooxidans |
EC Number | Storage Stability | Organism |
---|---|---|
7.1.2.2 | 4°C, Tris-SO4 buffer, pH 8.5, 8 days | Acidithiobacillus ferrooxidans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.2.2 | ATP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | ADP + phosphate + H+/out | - |
? | |
7.1.2.2 | GTP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | GDP + phosphate + H+/out | - |
? | |
7.1.2.2 | ITP + H2O + H+/in | - |
Acidithiobacillus ferrooxidans | IDP + phosphate + H+/out | - |
? | |
7.1.2.2 | additional information | no hydrolysis of UTP nor ADP | Acidithiobacillus ferrooxidans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.1.2.2 | More | x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1 | Acidithiobacillus ferrooxidans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.2.2 | F1-ATPase | - |
Acidithiobacillus ferrooxidans |
7.1.2.2 | F1F0-ATPase | - |
Acidithiobacillus ferrooxidans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.1.2.2 | 45 | - |
- |
Acidithiobacillus ferrooxidans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.1.2.2 | 8.5 | - |
- |
Acidithiobacillus ferrooxidans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
7.1.2.2 | 7 | 9.5 | ATPase activity decreases markedly above or below this range | Acidithiobacillus ferrooxidans |