EC Number | Application | Comment | Organism |
---|---|---|---|
5.4.99.5 | drug development | multiple-drug-resistant tuberculoses bacillus and its synergism with HIV-characterization of new enzyme targets and the development of new drugs | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.4.99.5 | MtCM (Rv1885c) (PDB ID-2F6L), docked conformation of (3S,6Z)-8-hydroxy-2-oxabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid, 4-[[2-(3,4-dimethoxyphenyl)ethyl]amino]-3-nitro-5-sulfamoylbenzoic acid, and (3S,6Z)-8-hydroxy-2-azabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid, (2Z)-2-(4-chlorophenyl)-3-(4,5-dimethoxy-2-nitrophenyl)prop-2-enoic acid in the catalytic site of MtCM x-ray crystal structure is shown | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.5 | (2Z)-2-(4-chlorophenyl)-3-(4,5-dimethoxy-2-nitrophenyl)prop-2-enoic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | (3R,6Z)-8-hydroxy-2-azabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | (3S,6Z)-8-hydroxy-2-azabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | (3S,6Z)-8-hydroxy-2-oxabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | 4-[[2-(3,4-dimethoxyphenyl)ethyl]amino]-3-nitro-5-sulfamoylbenzoic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | methyl 4-(methylamino)-3-nitrobenzoate | - |
Mycobacterium tuberculosis | |
5.4.99.5 | additional information | aza inhibitors. Competitive inhibition, Saccharomyces cerevisiae chorismate mutase inhibitors and the substrate chorismic acid used for pharmacophore model generation. These inhibitors do not alter Vmax at the higher concentration of substrate (1-5 mM) | Mycobacterium tuberculosis | |
5.4.99.5 | N-[[3-(tert-butoxycarbonyl)-2-phenyl-1,3-thiazolidin-4-yl]carbonyl]leucine | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.5 | additional information | - |
Chorismic acid | Competetive inhibition by I IV Structur: increase the Km | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.4.99.5 | extracellular | MtCM is secreted out of the cell to provide support to Mycobacterium tuberculosis in aromatic amino acid deficient medium | Mycobacterium tuberculosis | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.5 | Chorismate | Mycobacterium tuberculosis | - |
Prephenate | - |
? | |
5.4.99.5 | Chorismate | Mycobacterium tuberculosis H37Rv | - |
Prephenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.5 | Mycobacterium tuberculosis | P9WIB9 | - |
- |
5.4.99.5 | Mycobacterium tuberculosis H37Rv | P9WIB9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.5 | Chorismate | - |
Mycobacterium tuberculosis | Prephenate | - |
? | |
5.4.99.5 | Chorismate | - |
Mycobacterium tuberculosis H37Rv | Prephenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.5 | chorismate mutase | - |
Mycobacterium tuberculosis |
5.4.99.5 | MtCM | - |
Mycobacterium tuberculosis |
5.4.99.5 | Mycobacterium tuberculosis H37Rv chorismate mutase | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.5 | 6.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.5 | 0.0057 | - |
4-[[2-(3,4-dimethoxyphenyl)ethyl]amino]-3-nitro-5-sulfamoylbenzoic acid | 4-[[2-(3,4-dimethoxyphenyl)ethyl]amino]-3-nitro-5-sulfamoylbenzoic acid shows the most potent inhibition with Ki value of 0.0057 mM against MtCM | Mycobacterium tuberculosis | |
5.4.99.5 | 0.01771 | - |
(2Z)-2-(4-chlorophenyl)-3-(4,5-dimethoxy-2-nitrophenyl)prop-2-enoic acid | - |
Mycobacterium tuberculosis | |
5.4.99.5 | 0.0211 | - |
N-[[3-(tert-butoxycarbonyl)-2-phenyl-1,3-thiazolidin-4-yl]carbonyl]leucine | - |
Mycobacterium tuberculosis | |
5.4.99.5 | 0.0288 | - |
methyl 4-(methylamino)-3-nitrobenzoate | - |
Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
5.4.99.5 | 0.007 | - |
(3S,6Z)-8-hydroxy-2-oxabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | Mycobacterium tuberculosis | Saccharomyces cerevisiae chorismate mutase inhibitors | |
5.4.99.5 | 1.2 | - |
(3S,6Z)-8-hydroxy-2-azabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | Mycobacterium tuberculosis | Saccharomyces cerevisiae chorismate mutase inhibitors | |
5.4.99.5 | 18 | - |
(3R,6Z)-8-hydroxy-2-azabicyclo[3.3.3]undec-6-ene-3,5-dicarboxylic acid | Mycobacterium tuberculosis | Saccharomyces cerevisiae chorismate mutase inhibitors |