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Literature summary extracted from

  • Kim, B.; Hyun, Y.J.; Lee, K.S.; Kobashi, K.; Kim, D.H.
    Cloning, expression and purification of arylsulfate sulfotransferase from Eubacterium A-44 (2007), Biol. Pharm. Bull., 30, 11-14.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22 gene astA, cloned from a genomic library, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TH2, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Eubacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 75000
-
 x * 75000, cleaved recombinant enzyme, SDS-PAGE Eubacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Eubacterium sp.
-
-
-
2.8.2.22 Eubacterium sp. A-44
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by treatement with enterokinase to exclude the DsbA and nickel affinity chromatography Eubacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.22 25.6
-
 purified, recombinant, enterokinase-cleaved enzyme Eubacterium sp.
2.8.2.22 37.1
-
 purified recombinant enzyme Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + 4-hydroxybenzoate low activity Eubacterium sp. 4-nitrophenol + 4-hydroxybenzoyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-hydroxybenzoate low activity Eubacterium sp. A-44 4-nitrophenol + 4-hydroxybenzoyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine best acceptor substrate Eubacterium sp. 4-nitrophenol + tyramine sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine best acceptor substrate Eubacterium sp. A-44 4-nitrophenol + tyramine sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyrosine low activity Eubacterium sp. 4-nitrophenol + tyrosine sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyrosine low activity Eubacterium sp. A-44 4-nitrophenol + tyrosine sulfate
-
 ?
2.8.2.22 additional information the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors Eubacterium sp. ?
-
 ?
2.8.2.22 additional information the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors Eubacterium sp. A-44 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 ? x * 75000, cleaved recombinant enzyme, SDS-PAGE Eubacterium sp.

Synonyms

EC Number Synonyms Comment Organism
2.8.2.22 arylsulfate sulfotransferase
-
 Eubacterium sp.
2.8.2.22 ASST
-
 Eubacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.22 37
-
 assay at Eubacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.22 8
-
 assay at Eubacterium sp.