EC Number | General Stability | Organism |
---|---|---|
2.3.1.45 | Neu5Ac or CMP-Neu5Ac are strongly required for stabilization of the solubilized enzyme during purification | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.45 | CoA | competitive inhibitor; strong product inhibition | Bos taurus | |
2.3.1.45 | KCl | activates, optimally at 50 mM, 80% inhibition at 500 mM | Bos taurus | |
2.3.1.45 | additional information | no inhibition by AMP, CMP, EDTA and DTT | Bos taurus | |
2.3.1.45 | NaCl | 50% inhibition at 50 mM | Bos taurus | |
2.3.1.45 | Zn2+ | 50% inhibition at 1 mM | Bos taurus | |
2.3.1.45 | ZnCl2 | 50% inhibition at 1 mM | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.45 | additional information | - |
additional information | kinetics for the solubilized and the membrane-bound enzyme at different conditions, overview | Bos taurus | |
2.3.1.45 | 0.0073 | - |
CMP-N-acetylneuraminic acid | pH 6.5, 37°C, solubilized enzyme | Bos taurus | |
2.3.1.45 | 0.008 | - |
acetyl-CoA | pH 6.5, 37°C, solubilized enzyme | Bos taurus | |
2.3.1.45 | 0.0084 | - |
acetyl-CoA | - |
Bos taurus | |
2.3.1.45 | 0.1285 | - |
CMP-N-acetylneuraminic acid | - |
Bos taurus | |
2.3.1.45 | 0.1385 | - |
N-acetylneuraminic acid | - |
Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.45 | membrane | - |
Bos taurus | 16020 | - |
2.3.1.45 | microsome | - |
Bos taurus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.45 | KCl | activates, optimally at 50 mM for the solubilized enzyme and at 70 mM for the membrane-bound enzyme, 80% inhibition at 500 mM | Bos taurus | |
2.3.1.45 | KCl | optimum activity at 50 mM | Bos taurus | |
2.3.1.45 | additional information | the enzyme is not affected Mn2+, Ca2+, and Mg2+ | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.45 | 150000 | - |
between 150000-160000 Da, determined by gel filtration | Bos taurus |
2.3.1.45 | 150000 | 160000 | gel filtration | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.45 | additional information | Bos taurus | the O-acetylation of sialic acids in various positions is a frequent modification of these residues in glycoproteins and glycolipids, sialic acid O-acetylation is involved in the regulation of many cell biological and pathophysiological events | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.45 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.45 | native enzyme 53fold, partially, from microsomes by solubilization with the zwitterionic detergent CHAPS, i.e. 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonic acid, dialysis, anion exchange chromatography, and two steps of CoA affinity chromatography | Bos taurus |
2.3.1.45 | using gel filtration, ion exchange chromatography, and affinity chromatography | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.45 | submandibular gland | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.45 | -20°C, partially purified solubilized enzyme, 2 weeks, loss of 25% activity | Bos taurus |
2.3.1.45 | -80°C, partially purified solubilized and membrane-bound enzyme, stable for several months | Bos taurus |
2.3.1.45 | -80°C, stable for several months | Bos taurus |
2.3.1.45 | 22°C, room temperature, partially purified solubilized enzyme, almost complete loss of activity within a few hours | Bos taurus |
2.3.1.45 | 4°C, partially purified solubilized enzyme, 8 h, quite stable | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.45 | acetyl-CoA + CMP-N-acetylneuraminic acid | best substrate | Bos taurus | CoA + CMP-N-acetyl-9-O-acetyl-neuraminate | the 9-O-acetylsialic acid is the main product | ? | |
2.3.1.45 | acetyl-CoA + N-acetylneuraminic acid | - |
Bos taurus | CoA + N-acetyl-9-O-acetyl-neuraminate | the 9-O-acetylsialic acid is the main product | ? | |
2.3.1.45 | CMP-N-acetylneuraminic acid + acetyl-CoA | - |
Bos taurus | CoA + CMP-5-N-acetyl-9-O-acetyl neuraminic acid | - |
? | |
2.3.1.45 | additional information | the O-acetylation of sialic acids in various positions is a frequent modification of these residues in glycoproteins and glycolipids, sialic acid O-acetylation is involved in the regulation of many cell biological and pathophysiological events | Bos taurus | ? | - |
? | |
2.3.1.45 | additional information | substrate specificity, overview, no involvement of an isomerase in the migration of O-acetyl groups within the sialic acid side chain, at pH 9.5 in buffer only, a significant proportion of Neu5,7Ac2 is converted non-enzymatically to Neu5,9Ac within 15 min | Bos taurus | ? | - |
? | |
2.3.1.45 | N-acetylneuraminic acid + acetyl-CoA | - |
Bos taurus | CoA + 5-N-acetyl-9-O-acetyl neuraminic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.45 | sialate O-acetyltransferase | - |
Bos taurus |
2.3.1.45 | sialate-O-acetyltransferase | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.45 | 37 | - |
- |
Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.45 | additional information | - |
the solubilised enzyme loses approximately 25% of its activity after 2 weeks at -20°C. It is also stable at 48°C for 8 h, but loses most of its activity at room temperature within a few hours | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.45 | 6.5 | - |
- |
Bos taurus |
2.3.1.45 | 6.5 | - |
solubilized enzyme | Bos taurus |
2.3.1.45 | 7.5 | - |
membrane-bound enzyme | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.45 | acetyl-CoA | - |
Bos taurus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.45 | 0.02 | - |
CoA | - |
Bos taurus | |
2.3.1.45 | 0.02 | - |
CoA | pH 7.5, 37°C, microsome-bound enzyme | Bos taurus |