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Literature summary extracted from

  • Bonete, M.J.; Ferrer, J.; Pire, C.; Penades, M.; Ruiz, J.L.
    2-Hydroxyacid dehydrogenase from Haloferax mediteranii, a D-isomer-specific member of the 2-dehydrogenase family (2000), Biochimie, 82, 1143-1150.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.345 0.05
-
 NADH with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.086
-
 NADH with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.096
-
 NADH with pyruvate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.14
-
 D-lactate in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.36
-
 2-oxobutyrate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.56
-
 pyruvate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 2.3
-
 2-oxoisocaproate in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.345 NaCl maximum activity in the presence of 4 M NaCl Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.345 59500
-
 2 * 59500, CTAB-PAGE Haloferax mediterranei
1.1.1.345 62900
-
 2 * 62900, SDS-PAGE Haloferax mediterranei
1.1.1.345 101400
-
 gel filtration Haloferax mediterranei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.345 Haloferax mediterranei
-
-
-
1.1.1.345 Haloferax mediterranei R4, ATCC 33500
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.345 Sepharose column chromatography, DEAE-cellulose column chromatography, Q-Sepharose column chromatography, Sephacryl S-300 gel filtration, and Sephadex G25 gel filtration Haloferax mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.345 0.427
-
 with 2-oxoisocaproate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 0.799
-
 with 2-oxobutyrate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei
1.1.1.345 1.462
-
 with pyruvate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.345 2-oxobutyrate + NADH + H+ 55% activity compared to pyruvate Haloferax mediterranei D-2-hydroxybutyrate + NAD+
-
 r
1.1.1.345 2-oxobutyrate + NADH + H+ 55% activity compared to pyruvate Haloferax mediterranei R4, ATCC 33500 D-2-hydroxybutyrate + NAD+
-
 r
1.1.1.345 2-oxoisocaproate + NADH + H+ 29% activity compared to pyruvate Haloferax mediterranei L-2-hydroxyisocaproate + NAD+
-
 r
1.1.1.345 2-oxoisocaproate + NADH + H+ 29% activity compared to pyruvate Haloferax mediterranei R4, ATCC 33500 L-2-hydroxyisocaproate + NAD+
-
 r
1.1.1.345 D-2-hydroxybutyrate + NAD+
-
 Haloferax mediterranei 2-oxobutyrate + NADH + H+
-
 r
1.1.1.345 D-2-hydroxybutyrate + NAD+
-
 Haloferax mediterranei R4, ATCC 33500 2-oxobutyrate + NADH + H+
-
 r
1.1.1.345 D-lactate + NAD+
-
 Haloferax mediterranei pyruvate + NADH + H+
-
 r
1.1.1.345 L-2-hydroxycaproate + NAD+
-
 Haloferax mediterranei 2-oxocaproate + NADH + H+
-
 r
1.1.1.345 additional information no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate Haloferax mediterranei ?
-
 ?
1.1.1.345 additional information no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate Haloferax mediterranei R4, ATCC 33500 ?
-
 ?
1.1.1.345 pyruvate + NADH + H+ 100% activity Haloferax mediterranei D-lactate + NAD+
-
 r
1.1.1.345 pyruvate + NADH + H+ 100% activity Haloferax mediterranei R4, ATCC 33500 D-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.345 homodimer 2 * 59500, CTAB-PAGE Haloferax mediterranei
1.1.1.345 homodimer 2 * 62900, SDS-PAGE Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.345 NAD-dependent D-2-hydroxyacid dehydrogenase
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.345 52
-
-
 Haloferax mediterranei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.345 65 75 above 60°C there is a pronounced activity decrease, while there is nearly no activity at 75°C Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.345 5
-
 optimum pH for reaction with pyruvate or 2-oxobutyrate Haloferax mediterranei
1.1.1.345 7.5 8.5 optimum pH values for reaction with 2-oxoisocaproate Haloferax mediterranei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.345 5 9
-
 Haloferax mediterranei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.345 NAD+ the enzyme is strictly NAD+-dependent Haloferax mediterranei