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Literature summary extracted from

  • Gao, B.; Ellis, H.R.
    Mechanism of flavin reduction in the alkanesulfonate monooxygenase system (2007), Biochim. Biophys. Acta, 1774, 359-367.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.5 gene ssuD, the alkanesulfonate monooxygenase system, expressed from the ssuEADCB operon, is comprised of a flavin reductase encoded by ssuE and monooxygenase encoded by ssuD, ssuD expressionin strain BL21(DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.5 additional information
-
 additional information stopped-flow kinetics Escherichia coli
1.14.14.5 additional information
-
 additional information rapid reaction kinetic analysis of SsuE-catalyzed flavin reduction using NADPH Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.38 FMN + NADPH + H+ Escherichia coli
-
 FMNH2 + NADP+
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2 Escherichia coli the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation an aldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2 Escherichia coli the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview an aldehyde + FMN + sulfite + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.38 Escherichia coli
-
-
-
1.14.14.5 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.5 an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O kinetic reaction mechanism, charge transfer complex formation with flavin mononucleotide reductase in the alkanesulfonate monooxygenase system Escherichia coli
a
1.14.14.5 an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O mechanism of flavin reduction in the alkanesulfonate monooxygenase system, overview Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.38 FMN + NADPH + H+
-
 Escherichia coli FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ results from single-wavelength analyses at 450 and 550 nm show that reduction of FMN occurs in three distinct phases. Following a possible rapid equilibrium binding of FMN and NADPH to SsuE (MC-1) that occurs before the first detectable step, an initial fast phase (241 s-1) corresponds to the interaction of NADPH with FMN (CT-1). The second phase is a slow conversion (11 s-1) to form a charge-transfer complex of reduced FMNH2 with NADP+ (CT-2), and represents electron transfer from the pyridine nucleotide to the flavin. The third step (19 s-1) is the decay of the charge-transfer complex to SsuE with bound products (MC-2) or product release from the CT-2 complex. Results from isotope studies with [(4R)-2H]NADPH demonstrates a rate-limiting step in electron transfer from NADPH to FMN Escherichia coli FMNH2 + NADP+
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2
-
 Escherichia coli an aldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2 the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation Escherichia coli an aldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2 the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview Escherichia coli an aldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.5 an alkanesulfonate + FMNH2 + O2 mechanism of flavin reduction in the alkanesulfonate monooxygenase system, the FMN reductase, SsuE, catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase, SsuD, overview Escherichia coli an aldehyde + FMN + sulfite + H2O
-
 ?
1.14.14.5 additional information mechanism of flavin reduction in the alkanesulfonate monooxygenase system, consisting of the alkanesulfonate monooxygenase and the flavin mononucleotide reductase, which catalyzes the reduction of FMN by NADPH, overview Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.38 SsuE
-
 Escherichia coli
1.14.14.5 SsuD
-
 Escherichia coli
1.14.14.5 SsuE
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.5 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.5 FMNH2
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.5.1.38 physiological function FMN reductase (SsuE) catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase (SsuD) Escherichia coli