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Literature summary extracted from

  • Karyakin, A.; Motiejunas, D.; Wade, R.C.; Jung, C.
    FTIR studies of the redox partner interaction in cytochrome P450: the Pdx-P 450cam couple (2007), Biochim. Biophys. Acta, 1770, 420-431.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Iron heme iron Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O the active site structure changes due to putidaredoxin-enzyme interaction involving salt bridge and hydrogen bonding network between Arg112, His355, Leu356, and the heme ligand Cys357 Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin analysis of protein-protein interactions between enzyme and cofactor, overview Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.1 More structural changes upon complex formation between enzyme and cofactor cause an increase in beta-sheets and alpha-helix content, a decrease in population of random coil/3 10-helix structure, a redistribution of turn structures within the interacting proteins and changes in the protonation states or hydrogen-bondonh of amino acid carboxylic side chains Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 CYP101
-
 Pseudomonas putida
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin analysis of protein-protein interactions between enzyme and cofactor, structural changes upon complex formation, overview Pseudomonas putida