Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Toroser, D.; Yarian, C.S.; Orr, W.C.; Sohal, R.S.
    Mechanisms of gamma-glutamylcysteine ligase regulation (2006), Biochim. Biophys. Acta, 1760, 233-244.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 buthionine sulfoximine
-
 Drosophila melanogaster
6.3.2.2 MgATP2- although the enzyme preparation shows a strict requirement for MgATP2- for gamma-glutamylcysteine synthesis, preincubation of the homogenate under phosphorylating conditions with MgATP2- also causes a maximal inhibition of 89% Drosophila melanogaster
6.3.2.2 MgATP2- although the enzyme preparation shows a strict requirement for MgATP2- for gamma-glutamylcysteine synthesis, preincubation of the homogenates under phosphorylating conditions with MgATP2- also causes a maximal inhibition of 94%, 77%, 85%, 87%, 83% and 95% in cerebellum, hippocampus, brainstem, striatum, cortex and heart Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+ required Mus musculus
6.3.2.2 Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine Mus musculus the mechanism of modulation of eukaryotic gamma-glutamylcysteine ligase enzymes may include specific binding of ligands such as pyridine dinucleotide phosphates and reversible protein phosphorylation ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Drosophila melanogaster Q9W3K5
-
-
6.3.2.2 Mus musculus P97494
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.3.2.2 phosphoprotein the enzyme exists in vivo as a mixture of phosphorylated and dephosphorylated forms Mus musculus
6.3.2.2 phosphoprotein the enzyme exists in vivo as a mixture of phosphorylated and dephosphorylated forms Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 brain stem
-
 Mus musculus
-
6.3.2.2 cerebellum
-
 Mus musculus
-
6.3.2.2 heart
-
 Mus musculus
-
6.3.2.2 hippocampus
-
 Mus musculus
-
6.3.2.2 neostriatum
-
 Mus musculus
-
6.3.2.2 temporal lobe
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Drosophila melanogaster ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine the mechanism of modulation of eukaryotic gamma-glutamylcysteine ligase enzymes may include specific binding of ligands such as pyridine dinucleotide phosphates and reversible protein phosphorylation Mus musculus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 7.5
-
 in presence of NADPH Drosophila melanogaster
6.3.2.2 8.2
-
 in absence of NADPH Drosophila melanogaster