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Literature summary extracted from

  • Kang, H.K.; Seo, M.Y.; Seo, E.S.; Kim, D.; Chung, S.Y.; Kimura, A.; Day, D.F.; Robyt, J.F.
    Cloning and expression of levansucrase from Leuconostoc mesenteroides B-512 FMC in Escherichia coli (2005), Biochim. Biophys. Acta, 1727, 5-15.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Escherichia coli Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 AgNO3 1 mM, 86% inhibition Leuconostoc mesenteroides
2.4.1.10 FeSO4 1 mM, 17% inhibition Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 26.6
-
 sucrose
-
 Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 47100
-
 2 * 47100, calculated from sequence Leuconostoc mesenteroides
2.4.1.10 51700
-
 2 * 51700, enzyme including a His-tag, SDS-PAGE Leuconostoc mesenteroides
2.4.1.10 103000
-
 non-denaturing PAGE Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Leuconostoc mesenteroides Q5IS34
-
-
2.4.1.10 Leuconostoc mesenteroides B-512 FMC Q5IS34
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10
-
 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 126.6
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Leuconostoc mesenteroides glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Leuconostoc mesenteroides B-512 FMC glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 dimer 2 * 47100, calculated from sequence Leuconostoc mesenteroides
2.4.1.10 dimer 2 * 51700, enzyme including a His-tag, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
 optimal temperature for levan formation Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6.2
-
 levan synthesis Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 3.5 8.3 pH 3.5: about 55% of maximal activity, pH 8.3: about 60% of maximal activity, levan synthesis Leuconostoc mesenteroides