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Literature summary extracted from

  • Serov, A.E.; Odintzeva, E.R.; Uporov, I.V.; Tishkov, V.I.
    Use of Ramachandran plot for increasing thermal stability of bacterial formate dehydrogenase (2005), Biochemistry (Moscow), 70, 804-808.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 A191G non-optimal residue located by Ramachandran-plot, mutation has no significant effect on enzyme stability. No significant change in enzyme kinetic properties Pseudomonas sp.
1.17.1.9 H263G non-optimal residue located by Ramachandran-plot, mutation causes destabilization of enzyme and a 1.3fold increase in the monomolecular inactivation rate constant. No significant change in enzyme kinetic properties Pseudomonas sp.
1.17.1.9 N136G non-optimal residue located by Ramachandran-plot, mutation results in higher thermal stability and decreases the inactivation rate by 1.2fold. No significant change in enzyme kinetic properties Pseudomonas sp.
1.17.1.9 N234G non-optimal residue located by Ramachandran-plot, mutation has no significant effect on enzyme stability. No significant change in enzyme kinetic properties Pseudomonas sp.
1.17.1.9 Y144G non-optimal residue located by Ramachandran-plot, mutation results in higher thermal stability and decreases the inactivation rate by 1.4fold. 75% increase in Km-value of formate compared to wild-type Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Pseudomonas sp. P33160
-
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 62
-
 wild-type, monomolecular inactivation rate constant 25000 per sec Pseudomonas sp.