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Literature summary extracted from

  • Wu, H.; Min, J.; Ikeguchi, Y.; Zeng, H.; Dong, A.; Loppnau, P.; Pegg, A.E.; Plotnikov, A.N.
    Structure and mechanism of spermidine synthases (2007), Biochemistry, 46, 8331-8339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16 purified recombinant enzyme free or in complex with spermidine, putrescine, 5'-methyl, 5'-deoxymethylthioadenosine, and S-adenosylmethionine, hanging drop vapor diffusion method, 20°C, 10 mg/ml protein solution is mixed with reservoir solution, containing 20-25% PEG 3350, 0.1 M (NH4)2SO4, 0.1 M HEPES-NaOH, pH 7.5, and ligands in a ratio of 1: 5 enzyme-ligand, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.16 D101I site-directed mutagenesis, almost inactive mutant Thermotoga maritima
2.5.1.16 D170A site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme Thermotoga maritima
2.5.1.16 D173A site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme Thermotoga maritima
2.5.1.16 Y76F site-directed mutagenesis, the mutsnt shows reduced activity compared to the wild-type enzyme Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 S-Adenosyl-1,8-diamino-3-thiooctane
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 additional information
-
 additional information knetics of wild-type and mutant enzymes Thermotoga maritima
2.5.1.16 0.0075
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant enzyme Thermotoga maritima
2.5.1.16 0.009
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant enzyme Homo sapiens
2.5.1.16 0.019
-
 putrescine pH 7.5, 37°C, recombinant enzyme Thermotoga maritima
2.5.1.16 0.02
-
 putrescine pH 7.5, 37°C, recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosylmethioninamine + putrescine Homo sapiens
-
 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine Thermotoga maritima
-
 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Homo sapiens P19623
-
-
2.5.1.16 Thermotoga maritima Q9WZC2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine general mechanistic hypothesis for the aminopropyl transfer reaction Homo sapiens
a
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine general mechanistic hypothesis for the aminopropyl transfer reaction Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 5'-methyl,5'-deoxymethylthioadenosine + putrescine low activity Homo sapiens ?
-
 ?
2.5.1.16 5'-methyl,5'-deoxymethylthioadenosine + putrescine low activity Thermotoga maritima ?
-
 ?
2.5.1.16 additional information amine substrate specificity, overview Homo sapiens ?
-
 ?
2.5.1.16 additional information amine substrate specificity, overview Thermotoga maritima ?
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine
-
 Homo sapiens 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine
-
 Thermotoga maritima 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine the enzyme is highly specific for putrescine Homo sapiens 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine the enzyme shows a relatively low substrate specificity for putrescine Thermotoga maritima 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine low activity Homo sapiens spermidine + 5'-methylthioadenosine
-
 ?
2.5.1.16 S-adenosylmethioninamine + putrescine low activity Thermotoga maritima spermidine + 5'-methylthioadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 More structure analysis, comparison to the enzyme from Thermotoga maritima Homo sapiens
2.5.1.16 More structure analysis, comparison to the human enzyme Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 SPDSYN
-
 Homo sapiens
2.5.1.16 SPDSYN
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Homo sapiens
2.5.1.16 37 80 assay at Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.16 37 80
-
 Thermotoga maritima
2.5.1.16 37 50
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.16 80
-
 2 min, purified recombinant enzyme, stable Thermotoga maritima
2.5.1.16 80
-
 2 min, recombinant enzyme, inactivation Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.16 additional information
-
 additional information
-
 Thermotoga maritima
2.5.1.16 0.012
-
 putrescine pH 7.5, 80°C, recombinant mutant D101I Thermotoga maritima
2.5.1.16 0.012
-
 S-adenosylmethioninamine pH 7.5, 80°C, recombinant mutant D101I Thermotoga maritima
2.5.1.16 0.014
-
 putrescine pH 7.5, 80°C, recombinant mutant D170A Thermotoga maritima
2.5.1.16 0.014
-
 S-adenosylmethioninamine pH 7.5, 80°C, recombinant mutant D170A Thermotoga maritima
2.5.1.16 0.77
-
 putrescine pH 7.5, 37°C, recombinant wild-type enzyme Thermotoga maritima
2.5.1.16 0.77
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant wild-type enzyme Thermotoga maritima
2.5.1.16 1.5
-
 putrescine pH 7.5, 80°C, recombinant mutant D173A Thermotoga maritima
2.5.1.16 1.5
-
 S-adenosylmethioninamine pH 7.5, 80°C, recombinant mutant D173A Thermotoga maritima
2.5.1.16 1.9
-
 putrescine pH 7.5, 37°C, recombinant enzyme Homo sapiens
2.5.1.16 1.9
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant enzyme Homo sapiens
2.5.1.16 8.1
-
 putrescine pH 7.5, 80°C, recombinant mutant Y76F Thermotoga maritima
2.5.1.16 8.1
-
 S-adenosylmethioninamine pH 7.5, 80°C, recombinant mutant Y76F Thermotoga maritima
2.5.1.16 22.7
-
 putrescine pH 7.5, 80°C, recombinant wild-type enzyme Thermotoga maritima
2.5.1.16 22.7
-
 S-adenosylmethioninamine pH 7.5, 80°C, recombinant wild-type enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 7.5
-
 assay at Homo sapiens