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Literature summary extracted from

  • Herdendorf, T.J.; Miziorko, H.M.
    Phosphomevalonate kinase: functional investigation of the recombinant human enzyme (2006), Biochemistry, 45, 3235-3242.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.2 gene PMVK, DNA and amino acid sequence determination and analysis, functional expression of soluble His-tagged wild-type enzyme and of His-tagged mutants in Escherichia coli strains JM109 and BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.2 D23N site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
2.7.4.2 K17M site-directed mutagenesis, the mutant shows reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
2.7.4.2 K19M site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens
2.7.4.2 K22M site-directed mutagenesis, the mutant shows a 10000fold reduced activity in both forward and reverse reactions compared to the wild-type enzyme, almost inactive mutant Homo sapiens
2.7.4.2 R18Q site-directed mutagenesis, the mutant shows highly reduced activity in both forward and reverse reactions compared to the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 additional information
-
 additional information steady-state kinetics Homo sapiens
2.7.4.2 0.034
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.7.4.2 0.041
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.7.4.2 0.047
-
 ADP pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.7.4.2 0.047
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
2.7.4.2 0.065
-
 ADP pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
2.7.4.2 0.079
-
 ADP pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
2.7.4.2 0.079
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
2.7.4.2 0.107
-
 ATP pH 7.0, 30°C, recombinant wild-type enzyme Homo sapiens
2.7.4.2 0.107
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.4.2 0.116
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
2.7.4.2 0.123
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
2.7.4.2 0.131
-
 ADP pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
2.7.4.2 0.138
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
2.7.4.2 0.176
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
2.7.4.2 0.187
-
 (R)-mevalonate 5-diphosphate pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
2.7.4.2 0.23
-
 (R)-mevalonate 5-phosphate pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
2.7.4.2 0.286
-
 ADP pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
2.7.4.2 0.536
-
 ATP pH 7.0, 30°C, recombinant mutant R18Q Homo sapiens
2.7.4.2 0.536
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.4.2 0.556
-
 ATP pH 7.0, 30°C, recombinant mutant K17M Homo sapiens
2.7.4.2 0.556
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.4.2 0.815
-
 ATP pH 7.0, 30°C, recombinant mutant D23N Homo sapiens
2.7.4.2 0.815
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.4.2 1.312
-
 ATP pH 7.0, 30°C, recombinant mutant K19M Homo sapiens
2.7.4.2 1.312
-
 ATP ATP in form of MgATP2- Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Mg2+ as MgATP2- Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.2 22900
-
 recombinant His-tagged wild-type enzyme, gel filtration Homo sapiens
2.7.4.2 24200
-
 1 * 24200, sequence calculation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-mevalonate 5-phosphate Homo sapiens the enzyme catalyzes a key step in isoprenoid/sterol biosynthesis ADP + (R)-mevalonate 5-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Homo sapiens Q15126 gene PMVK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.2 ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate residue R22 is important for catalysis Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate
-
 Homo sapiens ADP + (R)-5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + (R)-mevalonate 5-phosphate
-
 Homo sapiens ADP + (R)-mevalonate 5-diphosphate
-
 r
2.7.4.2 ATP + (R)-mevalonate 5-phosphate the enzyme catalyzes a key step in isoprenoid/sterol biosynthesis Homo sapiens ADP + (R)-mevalonate 5-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.2 monomer 1 * 24200, sequence calculation Homo sapiens
2.7.4.2 More homology modeling for structure analysis, the model conforms to a nucleoside monophosphate kinase family fold, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.4.2 More the enzyme belongs to the nucleoside monophosphate, NMP kinase family Homo sapiens
2.7.4.2 PMK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.2 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.2 ADP
-
 Homo sapiens
2.7.4.2 ATP as MgATP2-, the enzyme possesses an N-terminal basic residue rich sequence, a possible Walker A ATP binding motif Homo sapiens