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Literature summary extracted from

  • Yahata, N.; Saitoh, T.; Takayama, Y.; Ozawa, K.; Ogata, H.; Higuchi, Y.; Akutsu, H.
    Redox interaction of cytochrome c3 with [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F (2006), Biochemistry, 45, 1653-1662.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.2.1 0.0022
-
mutant cytochrome c3 Y65A 25°C, pH 7.0, mutant cytochrome c3 Y65A Desulfovibrio vulgaris
1.12.2.1 0.0026
-
ferricytochrome c 25°C, pH 7.0, wild-type cytochrome c3 Desulfovibrio vulgaris
1.12.2.1 0.0027
-
mutant cytochrome c3 K10M 25°C, pH 7.0, mutant cytochrome c3 K10M Desulfovibrio vulgaris
1.12.2.1 0.003
-
mutant cytochrome c3 K26M 25°C, pH 7.0, mutant cytochrome c3 K26M Desulfovibrio vulgaris
1.12.2.1 0.0043
-
mutant cytochrome c3 Y66L 25°C, pH 7.0, mutant cytochrome c3 Y66L Desulfovibrio vulgaris
1.12.2.1 0.0045
-
mutant cytochrome c3 K15M 25°C, pH 7.0, mutant cytochrome c3 K15M Desulfovibrio vulgaris
1.12.2.1 0.0051
-
mutant cytochrome c3 K57M 25°C, pH 7.0, mutant cytochrome c3 K57M Desulfovibrio vulgaris
1.12.2.1 0.0057
-
mutant cytochrome c3 K58M 25°C, pH 7.0, mutant cytochrome c3 K58M Desulfovibrio vulgaris
1.12.2.1 0.0057
-
mutant cytochrome c3 K94M 25°C, pH 7.0, mutant cytochrome c3 K94M Desulfovibrio vulgaris
1.12.2.1 0.0067
-
mutant cytochrome c3 K72M 25°C, pH 7.0, mutant cytochrome c3 K72M Desulfovibrio vulgaris
1.12.2.1 0.0073
-
mutant cytochrome c3 K95M 25°C, pH 7.0, mutant cytochrome c3 K95M Desulfovibrio vulgaris
1.12.2.1 0.0099
-
mutant cytochrome c3 K101M 25°C, pH 7.0, mutant cytochrome c3 K101M Desulfovibrio vulgaris
1.12.2.1 0.0104
-
mutant cytochrome c3 K60M 25°C, pH 7.0, mutant cytochrome c3 K60M Desulfovibrio vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.12.2.1 Desulfovibrio vulgaris
-
-
-
1.12.2.1 Desulfovibrio vulgaris Miyazaki F
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.2.1 H2 + ferricytochrome c
-
Desulfovibrio vulgaris H+ + ferrocytochrome c
-
?
1.12.2.1 H2 + ferricytochrome c
-
Desulfovibrio vulgaris Miyazaki F H+ + ferrocytochrome c
-
?
1.12.2.1 H2 + ferricytochrome c3 the positive charges of Lys60, Lys72, Lys95, and Lys101 around heme 4 are important for formation of the transient complex with [NiFe] hydrogenase in the initial stage of the cytochrome c3 reduction. Reaction with wild-type and mutant cytochrome c3 Desulfovibrio vulgaris H+ + ferrocytochrome c3
-
?
1.12.2.1 H2 + ferricytochrome c3 the positive charges of Lys60, Lys72, Lys95, and Lys101 around heme 4 are important for formation of the transient complex with [NiFe] hydrogenase in the initial stage of the cytochrome c3 reduction. Reaction with wild-type and mutant cytochrome c3 Desulfovibrio vulgaris Miyazaki F H+ + ferrocytochrome c3
-
?
1.12.2.1 H2 + mutant cytochrome c K101M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K15M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K26M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K57M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K58M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K60M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K72M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K94M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c K95M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c Y65A
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c Y66L
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K101M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K101M
-
Desulfovibrio vulgaris Miyazaki F ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K10M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K10M
-
Desulfovibrio vulgaris Miyazaki F ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K15M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K15M
-
Desulfovibrio vulgaris Miyazaki F ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K26M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K57M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K58M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K60M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K72M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K94M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 K95M
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 Y65A
-
Desulfovibrio vulgaris ?
-
r
1.12.2.1 H2 + mutant cytochrome c3 Y66L
-
Desulfovibrio vulgaris ?
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.2.1 42
-
mutant cytochrome c K72M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 44
-
mutant cytochrome c K26M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 45
-
mutant cytochrome c K15M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 45
-
mutant cytochrome c K58M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 45
-
mutant cytochrome c K94M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 45
-
mutant cytochrome c K95M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 46
-
mutant cytochrome c K60M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 47
-
mutant cytochrome c Y66L 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 48
-
mutant cytochrome c K57M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 48
-
mutant cytochrome c3 K10M 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 49
-
mutant cytochrome c Y65A 25°C, pH 7.0 Desulfovibrio vulgaris
1.12.2.1 49
-
ferricytochrome c 25°C, pH 7.0, wild-type cytochrome c3 Desulfovibrio vulgaris
1.12.2.1 51
-
mutant cytochrome c K101M 25°C, pH 7.0 Desulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.2.1 heme
-
Desulfovibrio vulgaris