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Literature summary extracted from

  • Horsman, G.P.; Ke, J.; Dai, S.; Seah, S.Y.; Bolin, J.T.; Eltis, L.D.
    Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway (2006), Biochemistry, 45, 11071-11086.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.7.1.8 wild-type, the S112C variant and S112C incubated with 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid by hanging drop vapor diffusion method at 20°C, to 1.6 A resolution, interaction between conserved active side residues and dienoate moiety of the substrate, the residue His265 is hydrogen-bonded to the 2-hydroxy/oxo substituent of the substrate, consistent with a role in catalyzing ketonization Paraburkholderia xenovorans

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.8 S112C lower enzyme activity Paraburkholderia xenovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.8 0.0003
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid wild-type at 3.2°C Paraburkholderia xenovorans
3.7.1.8 0.0028
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid S112C mutant at 25°C Paraburkholderia xenovorans

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Paraburkholderia xenovorans
-
 LB400
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid + H2O
-
 Paraburkholderia xenovorans benzoate + 2-hydroxypenta-2,4-dienoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase
-
 Paraburkholderia xenovorans
3.7.1.8 BphD
-
 Paraburkholderia xenovorans
3.7.1.8 HOPDA hydrolase
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.8 0.27
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid S112C mutant at 25°C Paraburkholderia xenovorans
3.7.1.8 0.98
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid wild-type at 3.2°C Paraburkholderia xenovorans
3.7.1.8 6.5
-
 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid wild-type at 25°C Paraburkholderia xenovorans