Literature summary extracted from

Barney, B.M.; Laryukhin, M.; Igarashi, R.Y.; Lee, H.I.; Dos Santos, P.C.; Yang, T.C.; Hoffman, B.M.; Dean, D.R.; Seefeldt, L.C.
Trapping a hydrazine reduction intermediate on the nitrogenase active site (2005), Biochemistry, 44, 8030-8037.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.18.6.1	V70A/H195Q	mutant used for freeze-trapping the FeMo-cofactor in a S=1/2 state with hydrazine as substrate. The trapped intermediate incorporates a hydrazine-derived species bound to the FeMo-cofactor. EPR and ENDOR analysis of the adduct	Azotobacter vinelandii

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.18.6.1	Iron	freeze-trapping the FeMo-cofactor in a S=1/2 state with hydrazine as substrate in mutant V70A/H195Q. The trapped intermediate incorporates a hydrazine-derived species bound to the FeMo-cofactor	Azotobacter vinelandii
1.18.6.1	Molybdenum	freeze-trapping the FeMo-cofactor in a S=1/2 state with hydrazine as substrate in mutant V70A/H195Q. The trapped intermediate incorporates a hydrazine-derived species bound to the FeMo-cofactor	Azotobacter vinelandii

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.18.6.1	Azotobacter vinelandii	-	-	-

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Release 2023.1 (January 2023)