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Literature summary extracted from

  • Saenger, A.K.; Nguyen, T.V.; Vockley, J.; Stankovich, M.T.
    Biochemical and electrochemical characterization of two variant human short-chain acyl-CoA dehydrogenases (2005), Biochemistry, 44, 16035-16042.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.8.1 additional information common variant SCAD enzymes and their potential contribution to clinical disease in humans Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1 into Escherichia coli XL1 Blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.1 G185S impairments of both its kinetic and electron transfer properties Homo sapiens
1.3.8.1 R147W exhibits almost identical physical and redox properties to wild-type but only half of the specific activity and substrate activation shifts observed in wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.055
-
 butyryl-CoA wild-type Homo sapiens
1.3.8.1 0.065
-
 butyryl-CoA R147W mutant Homo sapiens
1.3.8.1 0.124
-
 butyryl-CoA G185S mutant Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.1 44000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1 by gel filtration, to more than 95% purity Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.3.8.1 -80°C, phosphate buffer, pH 7.6, 20% glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor
-
 Homo sapiens 2-butenoyl-CoA + reduced acceptor
-
 ?
1.3.8.1 crotonyl-CoA + electron acceptor
-
 Homo sapiens butyryl-CoA + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.8.1 SCAD
-
 Homo sapiens
1.3.8.1 short-chain acyl-CoA dehydrogenase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.1 FAD
-
 Homo sapiens