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Literature summary extracted from

  • Kurz, L.C.; Fite, B.; Jean, J.; Park, J.; Erpelding, T.; Callis, P.
    Photophysics of tryptophan fluorescence: link with the catalytic strategy of the citrate synthase from Thermoplasma acidophilum (2005), Biochemistry, 44, 1394-1413.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.16 unliganded form and the enzyme in complex with oxaloacetate. Oxaloacetate is most likely the quencher of tryptophan fluorescence with the oxaloacetate carbonyl as the electron acceptor Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 H187Q analysis of tryptophan fluorescence Thermoplasma acidophilum
2.3.3.16 R344K analysis of tryptophan fluorescence Thermoplasma acidophilum
2.3.3.16 W245F/W115F/W17F analysis of tryptophan flourescence Thermoplasma acidophilum
2.3.3.16 W348Y analysis of tryptophan fluorescence, residue responsible for quenching effect Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0005
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.0005
-
 citrate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.00067
-
 acetyl-CoA wild-type, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.0007
-
 citrate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.00085
-
 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.001
-
 citrate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.003
-
 oxaloacetate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.003
-
 oxaloacetate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.003
-
 oxaloacetate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.0036
-
 acetyl-CoA mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.01
-
 acetyl-CoA mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.017
-
 acetyl-CoA mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.054
-
 oxaloacetate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.37
-
 oxaloacetate mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Thermoplasma acidophilum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Thermoplasma acidophilum citrate + CoA
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 0.03 0.55 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 0.5
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 6.73
-
 acetyl-CoA mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 7.75
-
 citrate mutant W348Y, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 9.8
-
 acetyl-CoA wild-type, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 11
-
 acetyl-CoA mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 11.4
-
 citrate wild-type, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 12.66
-
 citrate mutant H187Q, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 14.5
-
 acetyl-CoA mutant R344K, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 15.4
-
 acetyl-CoA mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum
2.3.3.16 17.3
-
 citrate mutant W245F/W115F/W17F, pH 8.0, 20°C Thermoplasma acidophilum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.3.16 Thermoplasma acidophilum mutant W245F/W115F/W17F, isoelectric focusing
-
 8.6
2.3.3.16 Thermoplasma acidophilum isoelectric focusing, wild-type
-
 8.8
2.3.3.16 Thermoplasma acidophilum mutant H187Q, isoelectric focusing
-
 8.8
2.3.3.16 Thermoplasma acidophilum mutant H222Q, isoelectric focusing
-
 8.8
2.3.3.16 Thermoplasma acidophilum mutant W348Y, isoelectric focusing
-
 8.8