Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roma, G.W.; Crowley, L.J.; Davis, C.A.; Barber, M.J.
    Mutagenesis of glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase (2005), Biochemistry, 44, 13467-13476.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 G179A mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
1.6.2.2 G179P mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
1.6.2.2 G179T mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus
1.6.2.2 G179V mutant preceeding the 180GxGxxP185 motif bindin the adenosine moiety of NAD(P)H. Incorporation of FAD and adsortion and CD spectra similar to wild-type. Decrease in NADH:ferricyanide activity and affinity for NADH Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.001
-
 ferrocytochrome b5 mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 0.006
-
 NADH wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 0.007
-
 ferricyanide mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 0.007
-
 ferricyanide mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 0.008
-
 ferricyanide wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 0.008
-
 ferricyanide mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 0.008
-
 ferrocytochrome b5 mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 0.008
-
 ferricyanide mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 0.013
-
 ferrocytochrome b5 wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 0.025
-
 NADH mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 0.043
-
 ferrocytochrome b5 mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 0.107
-
 ferrocytochrome b5 mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 0.375
-
 NADPH mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 0.507
-
 NADPH mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 0.595
-
 NADH mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 0.662
-
 NADH mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 0.924
-
 NADPH wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 1.077
-
 NADH mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 1.36
-
 NADPH mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 2.317
-
 NADPH mutant G179P, pH 7.0 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Rattus norvegicus P20070
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricyanide + NADH
-
 Rattus norvegicus 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.2.2 2 ferricyanide + NADPH
-
 Rattus norvegicus 2 ferrocyanide + NADP+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 8
-
 NADPH mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 10
-
 ferrocytochrome b5 mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 12
-
 NADPH mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 12
-
 ferricyanide mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 17
-
 NADPH mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 17
-
 ferrocytochrome b5 mutant G179V, pH 7.0 Rattus norvegicus
1.6.2.2 18
-
 ferrocytochrome b5 mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 33
-
 NADPH wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 33
-
 ferricyanide mutant G179T, pH 7.0 Rattus norvegicus
1.6.2.2 42
-
 ferricyanide mutant G179P, pH 7.0 Rattus norvegicus
1.6.2.2 48
-
 NADPH mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 245
-
 ferrocytochrome b5 mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 400
-
 ferrocytochrome b5 wild-type, pH 7.0 Rattus norvegicus
1.6.2.2 559
-
 ferricyanide mutant G179A, pH 7.0 Rattus norvegicus
1.6.2.2 800
-
 ferricyanide wild-type, pH 7.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Rattus norvegicus
1.6.2.2 NADPH
-
 Rattus norvegicus