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Literature summary extracted from

  • Wang, H.; Liu, L.; Hanna, P.E.; Wagner, C.R.
    Catalytic mechanism of hamster arylamine N-acetyltransferase 2 (2005), Biochemistry, 44, 11295-11306.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 expression of wild-type and mutant NAT2 in Escherichia coli, the mutant variants are insoluble, inactive enzymes Mesocricetus auratus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.5 H107N site-directed mutagenesis, no expression in Escherichia coli cells possible Mesocricetus auratus
2.3.1.5 H107Q site-directed mutagenesis, no expression in Escherichia coli cells possible, insoluble inactive enzyme Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 additional information
-
 additional information steady-state kinetics, kinetic and isotope effect studies Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Mesocricetus auratus
-
 CoA + an N-acetylarylamine
-
 ?
2.3.1.5 additional information Mesocricetus auratus the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Mesocricetus auratus P50293 isozyme NAT2; isozyme NAT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 recombinant wild-type and mutant NAT2 from Escherichia coli by anion exchange chromatography Mesocricetus auratus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.5 acetyl-CoA + an arylamine = CoA + an N-acetylarylamine ping pong reaction mechanism, the enzyme contains a strictly conserved a Cys-His-Asp catalytic triad, acetylation of the hamster NAT2 is dependent on a thiolate-imidazolium ion pair, Cys-S--His-ImH+, and not a general acid-base catalysis, a deacetylation mechanism dominated by nucleophilic attack of the thiol ester for arylamines Mesocricetus auratus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.5 recovery of 25-50% activity of the recombinant wild-type enzyme after expression in Escherichia coli Mesocricetus auratus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
-
 Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-nitroaniline
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-nitroaniline
-
 ?
2.3.1.5 4-nitrophenyl acetate + anisidine
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-anisidine
-
 ?
2.3.1.5 4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate
-
 Mesocricetus auratus 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid preferred substrate Mesocricetus auratus CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoyl-L-glutamic acid
-
 Mesocricetus auratus CoA + 4-acetylaminobenzoyl-L-glutamic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-nitroaniline
-
 Mesocricetus auratus CoA + N-acetyl-4-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + an arylamine
-
 Mesocricetus auratus CoA + an N-acetylarylamine
-
 ?
2.3.1.5 acetyl-CoA + anisidine
-
 Mesocricetus auratus CoA + N-acetyl-anisidine
-
 ?
2.3.1.5 additional information the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics Mesocricetus auratus ?
-
 ?
2.3.1.5 additional information 4-aminobenzoic acid is the preferred substrate Mesocricetus auratus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase 2
-
 Mesocricetus auratus
2.3.1.5 NAT
-
 Mesocricetus auratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 25
-
 assay at Mesocricetus auratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 0.6
-
 4-nitroaniline pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 76
-
 4-aminobenzoyl glutamic acid pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 120
-
 4-aminobenzoyl glutamic acid pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 200
-
 4-Aminobenzoic acid pH 7.0, 25°C, with cofactor acetyl-CoA, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 260
-
 anisidine pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 620
-
 4-Aminobenzoic acid pH 7.0, 25°C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7
-
 assay at Mesocricetus auratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.5 5.2 9 pH profiles with cofactors acetyl-CoA and 4-nitrophenyl acetate, overview Mesocricetus auratus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Mesocricetus auratus