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Literature summary extracted from

  • Kitmitto, A.; Myronova, N.; Basu, P.; Dalton, H.
    Characterization and structural analysis of an active particulate methane monooxygenase trimer from Methylococcus capsulatus (Bath) (2005), Biochemistry, 44, 10954-10965.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 cytoplasm sMMO Methylococcus capsulatus 5737
-
1.14.18.3 membrane bound, pMMO Methylococcus capsulatus 16020
-
1.14.18.3 additional information two distinct enzyme forms exist: a soluble cytoplasmic sMMO and a membrane-bound particulate pMMO Methylococcus capsulatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 copper required, activates Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.3 23000
-
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview Methylococcus capsulatus
1.14.18.3 26000
-
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview Methylococcus capsulatus
1.14.18.3 47000
-
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview Methylococcus capsulatus
1.14.18.3 390000
-
gel filtration Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2 Methylococcus capsulatus
-
methanol + acceptor + H2O
-
?
1.14.18.3 methane + reduced acceptor + H* + O2 Methylococcus capsulatus Bath
-
methanol + acceptor + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus Bath
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.3 stable and active native pMMO from membranes, by solubilization with 1% w/v CHAPS, removal of soluble proteins, gel filtration, anion exchange chromatography, and ultrafiltration Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.3 0.034
-
purified enzyme, substrates propylene and duroquinol Methylococcus capsulatus
1.14.18.3 0.114
-
membrane-bound enzyme, substrates propylene and duroquinol Methylococcus capsulatus
1.14.18.3 0.23
-
membrane-bound enzyme, substrates propylene and NADH Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
Methylococcus capsulatus methanol + acceptor + H2O
-
?
1.14.18.3 methane + reduced acceptor + H* + O2
-
Methylococcus capsulatus Bath methanol + acceptor + H2O
-
?
1.14.18.3 propene + reduced acceptor + H+ + O2
-
Methylococcus capsulatus 1,2-epoxypropane + acceptor + H2O
-
?
1.14.18.3 propene + reduced acceptor + H+ + O2
-
Methylococcus capsulatus Bath 1,2-epoxypropane + acceptor + H2O
-
?
1.14.18.3 propylene + duroquinol + O2
-
Methylococcus capsulatus propylene epoxide + duroquinone + H2O
-
?
1.14.18.3 propylene + duroquinol + O2
-
Methylococcus capsulatus Bath propylene epoxide + duroquinone + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 trimer 1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 particulate methane monooxygenase
-
Methylococcus capsulatus
1.14.18.3 pMMO
-
Methylococcus capsulatus
1.14.18.3 pMMO hydroxylase
-
Methylococcus capsulatus
1.14.18.3 pMMO-H
-
Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.3 45
-
assay at Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.3 7.2
-
assay at Methylococcus capsulatus