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Literature summary extracted from
- Characterization of Escherichia coli D-arabinose 5-phosphate isomerase encoded by kpsF: implications for group 2 capsule biosynthesis (2006), Biochem. J., 395, 427-432.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.13 | EDTA | - |
Escherichia coli |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.13 | overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.13 | Cd2+ | - |
Escherichia coli | |
| 5.3.1.13 | Cu2+ | - |
Escherichia coli | |
| 5.3.1.13 | Hg2+ | - |
Escherichia coli | |
| 5.3.1.13 | Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.13 | 0.3 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 5.3.1.13 | 0.35 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 5.3.1.13 | 0.57 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
| 5.3.1.13 | 0.61 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
| 5.3.1.13 | 0.64 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 5.3.1.13 | 1.2 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.13 | Ca2+ | weak activator | Escherichia coli | |
| 5.3.1.13 | Fe2+ | weak activator | Escherichia coli | |
| 5.3.1.13 | Mg2+ | weak activator | Escherichia coli | |
| 5.3.1.13 | Mn2+ | weak activator | Escherichia coli | |
| 5.3.1.13 | additional information | Ba2+, Co2+, Ni2+ have no effect | Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 122000 | - |
gel filtration | Escherichia coli |
| 5.3.1.13 | 133000 | - |
gel filtration | Escherichia coli |
| 5.3.1.13 | 145000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.13 | Escherichia coli | P45395 | CFT073 | - |
| 5.3.1.13 | Escherichia coli CFT073 | P45395 | CFT073 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.13 | of the recombinant protein by Q-Sepharose fast flow column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 25 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.1.13 | -80° | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| 5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| 5.3.1.13 | D-Arabinose 5-phosphate | A5P | Escherichia coli | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| 5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| 5.3.1.13 | D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| 5.3.1.13 | D-Arabinose 5-phosphate | A5P | Escherichia coli CFT073 | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | tetramer | 4 x 133909, MALDI-MS | Escherichia coli |
| 5.3.1.13 | tetramer | 4 x 35000, SDS-PAGE, MALDI-MS | Escherichia coli |
| 5.3.1.13 | tetramer | 4 x 35100, MALDI-MS | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | G-API | D-glucitol phosphotranferase operon related API | Escherichia coli |
| 5.3.1.13 | K-API | capsular K-antigen related API | Escherichia coli |
| 5.3.1.13 | KpsF | - |
Escherichia coli |
| 5.3.1.13 | KPSF protein | - |
Escherichia coli |
| 5.3.1.13 | L-API | LPS related API | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 7.75 | - |
- |
Escherichia coli |
| 5.3.1.13 | 8.25 | - |
- |
Escherichia coli |
| 5.3.1.13 | 8.4 | - |
- |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 7 | 8.5 | - |
Escherichia coli |
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