Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ferreira, P.; Medina, M.; Guillen, F.; Martinez, M.J.; Van Berkel, W.J.; Martinez, A.T.
    Spectral and catalytic properties of aryl-alcohol oxidase, a fungal flavoenzyme acting on polyunsaturated alcohols (2005), Biochem. J., 389, 731-738.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expression in Emericella nidulans under control of the fungal alc promoter Pleurotus eryngii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.7 3-Phenyl-1-propanol competitive Pleurotus eryngii
1.1.3.7 4-anisic acid competitive Pleurotus eryngii
1.1.3.7 4-methoxybenzylamine uncompetitive, pH-dependent inhibition, best at pH 8.0 Pleurotus eryngii
1.1.3.7 benzylmethyl ether competitive Pleurotus eryngii
1.1.3.7 chavicol competitive Pleurotus eryngii
1.1.3.7 phenol competitive Pleurotus eryngii
1.1.3.7 Toluene competitive Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 additional information
-
 additional information stopped-flow and steady-state kinetics Pleurotus eryngii
1.1.3.7 0.014
-
 3-chloro-4-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.027
-
 4-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.094
-
 2,4-hexadien-1-ol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.107
-
 3-Chlorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.132
-
 4-Chlorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.227
-
 3-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.54
-
 veratryl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.553
-
 4-fluorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.554
-
 3-fluorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.632
-
 benzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.708
-
 cinnamyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.831
-
 isovanillyl alcohol pH 6.0, 24°C Pleurotus eryngii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.7 benzyl alcohol + O2 Pleurotus eryngii
-
 benzaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus eryngii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.7 recombinant enzyme from Emericella nidulans by gel filtration and anion exchange chromatography Pleurotus eryngii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.7 an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, the enzyme does not thermodynamically stabilize a flavin semiquinone radical and forms no sulphite adduct Pleurotus eryngii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.7 additional information
-
-
 Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 2,4-hexadien-1-ol + O2 high activity Pleurotus eryngii 2,4-hexadienal + H2O2
-
 ?
1.1.3.7 3-anisyl alcohol + O2
-
 Pleurotus eryngii 3-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 3-chloro-4-anisyl alcohol + O2 high activity Pleurotus eryngii 3-chloro-4-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 3-chlorobenzyl alcohol + O2
-
 Pleurotus eryngii 3-chlorobenzyl aldehyde + H2O2
-
 ?
1.1.3.7 3-fluorobenzyl alcohol + O2 low activity Pleurotus eryngii 3-fluorobenzyl aldehyde + H2O2
-
 ?
1.1.3.7 4-anisyl alcohol + O2 best substrate Pleurotus eryngii 4-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 4-chlorobenzyl alcohol + O2
-
 Pleurotus eryngii 4-chlorobenzyl aldehyde + H2O2
-
 ?
1.1.3.7 4-fluorobenzyl alcohol + O2
-
 Pleurotus eryngii 4-fluorobenzyl aldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Pleurotus eryngii benzaldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2 the enzyme catalyzes two half-reactions: oxidation of benzyl alcohol with FAD cofactor, and reduction of O2 with reduced cofactor FADH2, overview Pleurotus eryngii benzaldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2
-
 Pleurotus eryngii cinnamyl aldehyde + H2O2
-
 ?
1.1.3.7 isovanillyl alcohol + O2
-
 Pleurotus eryngii isovanillyl aldehyde + H2O2
-
 ?
1.1.3.7 additional information 4-(hydroxymethyl)-benzoic acid is a poor substrate Pleurotus eryngii ?
-
 ?
1.1.3.7 veratryl alcohol + O2
-
 Pleurotus eryngii veratryl aldehyde + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 24
-
 assay at Pleurotus eryngii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.7 47
-
 90% of maximal activity within this range Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 6
-
 3-fluorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 15
-
 3-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 22
-
 3-Chlorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 30
-
 benzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 32
-
 4-fluorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 46
-
 3-chloro-4-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 51
-
 4-Chlorobenzyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 65
-
 cinnamyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 114
-
 veratryl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 119
-
 2,4-hexadien-1-ol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 127
-
 isovanillyl alcohol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 142
-
 4-anisyl alcohol pH 6.0, 24°C Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 6
-
-
 Pleurotus eryngii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.7 2 11
-
 Pleurotus eryngii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.7 6 9 very stable Pleurotus eryngii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD flavoenzyme, involved in the catalytic reaction Pleurotus eryngii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.7 additional information
-
 additional information inhibition kinetcis Pleurotus eryngii
1.1.3.7 0.08
-
 4-anisic acid pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.11
-
 chavicol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 0.25
-
 4-methoxybenzylamine pH 8.0, 24°C Pleurotus eryngii
1.1.3.7 0.75
-
 Toluene pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 1.35
-
 benzylmethyl ether pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 1.92
-
 phenol pH 6.0, 24°C Pleurotus eryngii
1.1.3.7 4.48
-
 3-Phenyl-1-propanol pH 6.0, 24°C Pleurotus eryngii