EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.27 | expressed in Escherichia coli | Morus alba |
2.5.1.112 | expressed in Escherichia coli BL21(DE3)pLysS cells | Morus alba |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.27 | D49A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | F120A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | F157A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | F93A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | Q255A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | W159A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | Y153A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | Y170A | retains 84% activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | Y217A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.27 | Y54A | almost complete loss of activity compared to the wild type enzyme | Morus alba |
2.5.1.112 | D49A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | F120A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | F157A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | F93A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | Q255A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | W159A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | Y153A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | Y170A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | Y170A | the mutant retains 84% of wild type activity | Morus alba |
2.5.1.112 | Y217A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
2.5.1.112 | Y54A | the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP | Morus alba |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.27 | 0.0374 | - |
dimethylallyl diphosphate | - |
Morus alba | |
2.5.1.27 | 0.0682 | - |
ADP | - |
Morus alba | |
2.5.1.112 | 0.0374 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba | |
2.5.1.112 | 0.0682 | - |
ADP | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.27 | MgCl2 | dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 | Morus alba | |
2.5.1.27 | MnCl2 | dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 | Morus alba | |
2.5.1.112 | Mg2+ | the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2 | Morus alba | |
2.5.1.112 | Mn2+ | the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2 | Morus alba |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 35810 | - |
calculated from sequence of cDNA | Morus alba |
2.5.1.112 | 35810 | - |
x * 35810, calculated from amino acid sequence | Morus alba |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.112 | dimethylallyl diphosphate + ADP | Morus alba | 100% activity | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + AMP | Morus alba | 6.6% activity compared to ADP | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | Morus alba | 82% activity compared to ADP | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.27 | Morus alba | Q08ET4 | mulberry | - |
2.5.1.112 | Morus alba | Q08ET4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.27 | Ni-chelate affinity column chromatography | Morus alba |
2.5.1.112 | Ni-chelate affinity column chromatography | Morus alba |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.27 | leaf | - |
Morus alba | - |
2.5.1.112 | leaf | - |
Morus alba | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.27 | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP | - |
? | |
2.5.1.27 | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate | Morus alba | trans-zeatin riboside 5'-triphosphate + diphosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + ADP | - |
Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + AMP | AMP is a poor substrate | Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + ATP | - |
Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + CDP | - |
Morus alba | ? | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + dADP | - |
Morus alba | diphosphate + N6-(dimethylallyl)dADP | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + dATP | - |
Morus alba | diphosphate + N6-(dimethylallyl)dATP | - |
ir | |
2.5.1.27 | dimethylallyl diphosphate + GDP | GDP is a poor substrate | Morus alba | ? | - |
ir | |
2.5.1.27 | geranyl diphosphate + ADP | geranyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-geranyl-ADP | - |
? | |
2.5.1.27 | isopentenyl diphosphate + ADP | isopentyl diphosphate is a very poor substrate | Morus alba | diphosphate + N6-isopentyl-ADP | - |
? | |
2.5.1.27 | additional information | farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT | Morus alba | ? | - |
? | |
2.5.1.112 | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP | 2.2% activity compared to dimethylallyl diphosphate and ADP | Morus alba | trans-zeatin riboside 5'-diphosphate + diphosphate | - |
? | |
2.5.1.112 | 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP | 1.4% activity compared to dimethylallyl diphosphate and ADP | Morus alba | trans-zeatin riboside 5'-triphosphate + diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ADP | 100% activity | Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + AMP | 6.6% activity compared to ADP | Morus alba | diphosphate + N6-(dimethylallyl)adenosine phosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | 82% activity compared to ADP | Morus alba | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + CDP | 30% activity compared to ADP | Morus alba | ? | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + dADP | 50% activity compared to ADP | Morus alba | ? | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + dATP | 36% activity compared to ADP | Morus alba | ? | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + GDP | 6.4% activity compared to ADP | Morus alba | ? | - |
? | |
2.5.1.112 | geranyl diphosphate + ADP | 1.2% activity compared to dimethylallyl diphosphate and ADP | Morus alba | ? | - |
? | |
2.5.1.112 | isopentenyl diphosphate + ADP | 0.3% activity compared to dimethylallyl diphosphate and ADP | Morus alba | ? | - |
? | |
2.5.1.112 | additional information | no activity with farnesyl diphosphate, dAMP, CMP, GMP, IMP, IDP, UMP, UDP, FAD and NADH | Morus alba | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.112 | ? | x * 35810, calculated from amino acid sequence | Morus alba |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.27 | adenylate isopentenyltransferase | - |
Morus alba |
2.5.1.27 | AIPT | - |
Morus alba |
2.5.1.112 | adenylate isopentenyltransferase | - |
Morus alba |
2.5.1.112 | AIPT | - |
Morus alba |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.27 | 0.0235 | - |
ADP | - |
Morus alba | |
2.5.1.27 | 0.0345 | - |
dimethylallyl diphosphate | - |
Morus alba | |
2.5.1.112 | 0.024 | - |
ADP | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba | |
2.5.1.112 | 0.035 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 6 | 7 | - |
Morus alba |
2.5.1.112 | 6 | 7 | - |
Morus alba |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.27 | ADP | - |
Morus alba | |
2.5.1.27 | AMP | - |
Morus alba | |
2.5.1.27 | ATP | - |
Morus alba | |
2.5.1.112 | ADP | preferred cofactor | Morus alba | |
2.5.1.112 | ATP | - |
Morus alba |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.112 | 0.344 | - |
ADP | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba | |
2.5.1.112 | 0.921 | - |
dimethylallyl diphosphate | in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C | Morus alba |