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Literature summary extracted from

  • Abe, I.; Tanaka, H.; Abe, T.; Noguchi, H.
    Enzymatic formation of unnatural cytokinin analogs by adenylate isopentenyltransferase from mulberry (2007), Biochem. Biophys. Res. Commun., 355, 795-800.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 expressed in Escherichia coli Morus alba
2.5.1.112 expressed in Escherichia coli BL21(DE3)pLysS cells Morus alba

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.27 D49A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 F120A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 F157A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 F93A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 Q255A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 W159A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 Y153A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 Y170A retains 84% activity compared to the wild type enzyme Morus alba
2.5.1.27 Y217A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.27 Y54A almost complete loss of activity compared to the wild type enzyme Morus alba
2.5.1.112 D49A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 F120A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 F157A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 F93A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 Q255A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 W159A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 Y153A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 Y170A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 Y170A the mutant retains 84% of wild type activity Morus alba
2.5.1.112 Y217A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba
2.5.1.112 Y54A the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP Morus alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.27 0.0374
-
dimethylallyl diphosphate
-
Morus alba
2.5.1.27 0.0682
-
ADP
-
Morus alba
2.5.1.112 0.0374
-
dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba
2.5.1.112 0.0682
-
ADP in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.27 MgCl2 dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 Morus alba
2.5.1.27 MnCl2 dependent on presence of divalent cation, addition of 2 mM MnCl2 is 1.3 times more effective than that of 10 mM MgCl2 Morus alba
2.5.1.112 Mg2+ the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2 Morus alba
2.5.1.112 Mn2+ the enzyme is dependent on presence of divalent cation, and addition of 2 mM MnCl2 is 1.3times more effective than that of 10 mM MgCl2 Morus alba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.27 35810
-
calculated from sequence of cDNA Morus alba
2.5.1.112 35810
-
x * 35810, calculated from amino acid sequence Morus alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.112 dimethylallyl diphosphate + ADP Morus alba 100% activity diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + AMP Morus alba 6.6% activity compared to ADP diphosphate + N6-(dimethylallyl)adenosine phosphate
-
?
2.5.1.112 dimethylallyl diphosphate + ATP Morus alba 82% activity compared to ADP diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Morus alba Q08ET4 mulberry
-
2.5.1.112 Morus alba Q08ET4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.27 Ni-chelate affinity column chromatography Morus alba
2.5.1.112 Ni-chelate affinity column chromatography Morus alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.27 leaf
-
Morus alba
-
2.5.1.112 leaf
-
Morus alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butenyl)ADP
-
?
2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate is a very poor substrate Morus alba trans-zeatin riboside 5'-triphosphate + diphosphate
-
?
2.5.1.27 dimethylallyl diphosphate + ADP
-
Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
ir
2.5.1.27 dimethylallyl diphosphate + AMP AMP is a poor substrate Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
ir
2.5.1.27 dimethylallyl diphosphate + ATP
-
Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
ir
2.5.1.27 dimethylallyl diphosphate + CDP
-
Morus alba ?
-
ir
2.5.1.27 dimethylallyl diphosphate + dADP
-
Morus alba diphosphate + N6-(dimethylallyl)dADP
-
ir
2.5.1.27 dimethylallyl diphosphate + dATP
-
Morus alba diphosphate + N6-(dimethylallyl)dATP
-
ir
2.5.1.27 dimethylallyl diphosphate + GDP GDP is a poor substrate Morus alba ?
-
ir
2.5.1.27 geranyl diphosphate + ADP geranyl diphosphate is a very poor substrate Morus alba diphosphate + N6-geranyl-ADP
-
?
2.5.1.27 isopentenyl diphosphate + ADP isopentyl diphosphate is a very poor substrate Morus alba diphosphate + N6-isopentyl-ADP
-
?
2.5.1.27 additional information farnesyl diphosphate, geranylgeranyl diphosphate, IDP and UDP are no substrates for Morus alba AIPT Morus alba ?
-
?
2.5.1.112 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP 2.2% activity compared to dimethylallyl diphosphate and ADP Morus alba trans-zeatin riboside 5'-diphosphate + diphosphate
-
?
2.5.1.112 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP 1.4% activity compared to dimethylallyl diphosphate and ADP Morus alba trans-zeatin riboside 5'-triphosphate + diphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + ADP 100% activity Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + AMP 6.6% activity compared to ADP Morus alba diphosphate + N6-(dimethylallyl)adenosine phosphate
-
?
2.5.1.112 dimethylallyl diphosphate + ATP 82% activity compared to ADP Morus alba diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + CDP 30% activity compared to ADP Morus alba ?
-
?
2.5.1.112 dimethylallyl diphosphate + dADP 50% activity compared to ADP Morus alba ?
-
?
2.5.1.112 dimethylallyl diphosphate + dATP 36% activity compared to ADP Morus alba ?
-
?
2.5.1.112 dimethylallyl diphosphate + GDP 6.4% activity compared to ADP Morus alba ?
-
?
2.5.1.112 geranyl diphosphate + ADP 1.2% activity compared to dimethylallyl diphosphate and ADP Morus alba ?
-
?
2.5.1.112 isopentenyl diphosphate + ADP 0.3% activity compared to dimethylallyl diphosphate and ADP Morus alba ?
-
?
2.5.1.112 additional information no activity with farnesyl diphosphate, dAMP, CMP, GMP, IMP, IDP, UMP, UDP, FAD and NADH Morus alba ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.112 ? x * 35810, calculated from amino acid sequence Morus alba

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 adenylate isopentenyltransferase
-
Morus alba
2.5.1.27 AIPT
-
Morus alba
2.5.1.112 adenylate isopentenyltransferase
-
Morus alba
2.5.1.112 AIPT
-
Morus alba

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.27 0.0235
-
ADP
-
Morus alba
2.5.1.27 0.0345
-
dimethylallyl diphosphate
-
Morus alba
2.5.1.112 0.024
-
ADP in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba
2.5.1.112 0.035
-
dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.27 6 7
-
Morus alba
2.5.1.112 6 7
-
Morus alba

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.27 ADP
-
Morus alba
2.5.1.27 AMP
-
Morus alba
2.5.1.27 ATP
-
Morus alba
2.5.1.112 ADP preferred cofactor Morus alba
2.5.1.112 ATP
-
Morus alba

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.112 0.344
-
ADP in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba
2.5.1.112 0.921
-
dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C Morus alba