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Literature summary extracted from

  • Sousa, S.A.; Moreira, L.M.; Wopperer, J.; Eberl, L.; Sa-Correia, I.; Leitao, J.H.
    The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities (2007), Biochem. Biophys. Res. Commun., 353, 200-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.13 expressed in Escherichia coli BL21(DE3) cells Burkholderia cepacia
2.7.7.13 gene bceA, the gene is part of the Burkholderia cepacia IST408 exopolysaccharide biosynthetic cluster, physical organization, unrooted phylogenetic tree, DNA and amino acid sequence determination, analysis, and comparisons, overview. Expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Burkholderia cepacia
5.3.1.8
-
 Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.8 additional information enzyme-deficient mutant is not impaired in synthesis of exopolysaccharide. However, the viscosity of aqueous solutions prepared with the exopolysaccharide produced by the mutant is significantly reduced compared with wild-type biopolymer and the mutant forms biofilms with a size reduced by 6fold Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 EDTA 2 mM, complete inhibition Burkholderia cepacia
5.3.1.8 additional information not inhibitory: mannose 1-phosphate, GTP, GDP-mannose, or diphosphate Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 2.9
-
 diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
2.7.7.13 2.9
-
 GDP-mannose pH 7.6, recombinant enzyme, presence of 10 mM Mn2+ Burkholderia cepacia
5.3.1.8 9
-
 D-mannose 6-phosphate pH 7.6, presence of 5 mM Mg2+ Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Ca2+ strongly dependent Burkholderia cepacia
2.7.7.13 Ca2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
2.7.7.13 Mn2+ strongly dependent Burkholderia cepacia
2.7.7.13 Mn2+ GMP activity is strongly dependent on the presence of Ca2+ or Mn2+ Burkholderia cepacia
2.7.7.13 additional information no activation and activity by Ni2+, Mg2+, and Zn2+ Burkholderia cepacia
5.3.1.8 Ca2+ best activator, may be replaced by Mg2+ or Mn2+ for phosphomannose isomerase activity Burkholderia cepacia
5.3.1.8 Mg2+ 60% of activity with Ca2+ for phosphomannose isomerase activity Burkholderia cepacia
5.3.1.8 Mn2+ 65% of activity with Ca2+ for phosphomannose isomerase activity Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 55000
-
 SDS-PAGE Burkholderia cepacia
5.3.1.8 55000
-
 x * 55300, calculated, x * 55000, SDS-PAGE Burkholderia cepacia
5.3.1.8 55300
-
 x * 55300, calculated, x * 55000, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 diphosphate + GDP-mannose Burkholderia cepacia
-
 GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 diphosphate + GDP-mannose Burkholderia cepacia IST408
-
 GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 additional information Burkholderia cepacia the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
 ?
2.7.7.13 additional information Burkholderia cepacia IST408 the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Burkholderia cepacia
-
-
-
2.7.7.13 Burkholderia cepacia Q06XM8 gene bceA
-
2.7.7.13 Burkholderia cepacia IST408
-
-
-
2.7.7.13 Burkholderia cepacia IST408 Q06XM8 gene bceA
-
5.3.1.8 Burkholderia cepacia Q06XM8 type II enzyme, bifunctional protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 Hi-Trap column chromatography Burkholderia cepacia
2.7.7.13 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 diphosphate + GDP-mannose
-
 Burkholderia cepacia GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 diphosphate + GDP-mannose
-
 Burkholderia cepacia IST408 GTP + alpha-D-mannose 1-phosphate
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Burkholderia cepacia diphosphate + GDP-alpha-D-mannose
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Burkholderia cepacia IST408 diphosphate + GDP-alpha-D-mannose
-
 ?
2.7.7.13 additional information the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cepacia ?
-
 ?
2.7.7.13 additional information the enzyme of Burkholderia cepacia, encoded by gene bceA, is bifunctional exhibiting phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities Burkholderia cepacia IST408 ?
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Burkholderia cepacia D-Fructose 6-phosphate
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Burkholderia cepacia IST408 D-Fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.8 ? x * 55300, calculated, x * 55000, SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
2.7.7.13 BceA bifunctional protein with phosphomannose isomerase and GDP-mannose pyrophosphorylase activities Burkholderia cepacia
2.7.7.13 GDP-D-mannose pyrophosphorylase
-
 Burkholderia cepacia
5.3.1.8 becA
-
 Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.13 6.85
-
 diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
2.7.7.13 195.9
-
 diphosphate in the presence of 10 mM Mn2+ Burkholderia cepacia
2.7.7.13 195.9
-
 GDP-mannose pH 7.6, recombinant enzyme, presence of 10 mM Mn2+ Burkholderia cepacia
5.3.1.8 19.4
-
 D-mannose 6-phosphate pH 7.6, presence of 5 mM Mg2+ Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 7.6
-
 assay at Burkholderia cepacia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.13 Burkholderia cepacia calculated from amino acid sequence
-
 6.03
5.3.1.8 Burkholderia cepacia calculated
-
 6