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Literature summary extracted from

  • Burnell, J.N.; Chastain, C.J.
    Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene (2006), Biochem. Biophys. Res. Commun., 345, 675-680.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.9.1 additional information the protein kinase/phosphatase RP catalyzes reversibly phosphorylation of T456, active form dephosphorylated at T456, diphosphate-forming dephosphorylation mechanism, assay at 25°C Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.27 His-tagged protein expressed in Escherichia coli NM522 Zea mays
2.7.9.1 expressed in Escherichia coli Zea mays
2.7.11.32 His-tagged protein expressed in Escherichia coli NM522 Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 additional information ADP-dependent inactivation assay of purified PPDK by bacterially-expressed RP described (47% inhibition of PPDK after 10min in presence of 2 mM ADP at 25°C), reversibility of RP activity by diphosphate-dependent reactivation assay described (1 mM diphosphate, at 25°C), SDS-PAGE and Western-Blot for detection of T456P-PPDK and RP Zea mays
2.7.9.1 PPDK regulatory protein RP catalyzes reversible phosphorylation on T456 by RP, inactive form phoshorylated at T456, ADP-dependent, a catalytic His-phophorylation precedes phosphorylation at T456, pyruvate (2 mM) can inhibit inactivation by RP Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.27 40000
-
 x * 40000, SDS-PAGE Zea mays
2.7.11.32 40000
-
 x * 40000, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Zea mays
-
-
-
2.7.9.1 Zea mays P11155
-
-
2.7.11.32 Zea mays
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.9.1 phosphoprotein phosphorylation/dephosphorylation by the PPDK regulatory protein RP at T456, RP-reaction described as PPDK-T456 + ADP = PPDK-T456P + AMP and PPDK-T456P + phophate = PPDK-T456 + diphosphate Zea mays

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.27 immobilized metal ion affinity chromatography (Ni2+) Zea mays
2.7.9.1 recombinant maize PPDK used for inactivation/activation assay by the PPDK regulatory protein RP Zea mays
2.7.11.32 immobilized metal ion affinity chromatography (Ni2+) Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.27 leaf
-
 Zea mays
-
2.7.9.1 leaf
-
 Zea mays
-
2.7.11.32 leaf
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 [pyruvate, phosphate dikinase] phosphate + phosphate Thr456 and His458 phosphorylated (inactive) Zea mays pyruvate, phosphate dikinase + diphosphate His458 phosphorylated (active) ?
2.7.11.32 pyruvate, phosphate dikinase + ADP His458 phosphorylated (active) Zea mays [pyruvate, phosphate dikinase] phosphate + AMP Thr456 and His458 phosphorylated (inactive) ?

Subunits

EC Number Subunits Comment Organism
2.7.4.27 ? x * 40000, SDS-PAGE Zea mays
2.7.11.32 ? x * 40000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.4.27 PPDK regulatory protein bifunctional serine/threonine kinase-phosphatase Zea mays
2.7.9.1 PPDK
-
 Zea mays
2.7.9.1 pyruvate orthophosphate dikinase
-
 Zea mays
2.7.9.1 pyruvate Pi dikinase
-
 Zea mays
2.7.11.32 PPDK regulatory protein bifunctional serine/threonine kinase-phosphatase Zea mays