Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Velasco-Garcia, R.; Zaldivar-Machorro, V.J.; Mujica-Jimenez, C.; Gonzalez-Segura, L.; Munoz-Clares, R.A.
    Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase--a potential target for antimicrobial agents against Pseudomonas aeruginosa (2006), Biochem. Biophys. Res. Commun., 341, 408-415.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.8 medicine betaine aldehyde dehydrogenase is a potential target for antimicrobial agents against Pseudomonas aeruginosa Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 wild type Pseudomonas aeruginosa betaine aldehyde dehydrogenase and the four mutants C353A, C377A, C439A and C286A Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 C286A cysteine 286 plays an important role in the maintenance ot the tetrameric structure Pseudomonas aeruginosa
1.2.1.8 C353A cysteine 353 not essential for enzyme activity Pseudomonas aeruginosa
1.2.1.8 C377A cysteine 377 not essential for enzyme activity Pseudomonas aeruginosa
1.2.1.8 C439A cysteine 439 not essential for enzyme activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 5,5'-dithiobis[2-nitrobenzoic acid] the effect of the thiol reagent DTNB on the native enzyme structure of the wild type enzyme and the mutants is examined Pseudomonas aeruginosa
1.2.1.8 bis[diethylthiocarbamyl]disulfide the effect of the thiol reagent disulfiram on the native enzyme structure of the wild type enzyme and the mutants is examined Pseudomonas aeruginosa
1.2.1.8 S-methylmethanesulfonate the effect of the thiol reagent MMTS on the native enzyme structure of the wild type enzyme and the mutants is examined Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NAD(P)H + 2 H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 purified to homogeneity following a two-step procedure Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NAD(P)H + 2 H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.8 tetramer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 betaine aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde:NAD(P)+ oxidoreductase
-
 Pseudomonas aeruginosa
1.2.1.8 PaBADH
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.8 30
-
 activity assay Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
 activity assay Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NADP+
-
 Pseudomonas aeruginosa