Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Beauvais, L.G.; Lippard, S.J.
    Reactions of the diiron(IV) intermediate Q in soluble methane monooxygenase with fluoromethanes (2005), Biochem. Biophys. Res. Commun., 338, 262-266.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 expression in Escherichia coli Methylococcus capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.25 additional information
-
 additional information soluble enzyme, stopped-flow transient kinetics, double-mixing stopped-flow optical spectroscopic measurements, difluoromethane exhibits a smaller second-order rate constant for reaction with intermediate Q compared to methane Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2 Methylococcus capsulatus Bath via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane methanol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
-
-
1.14.13.25 Methylococcus capsulatus Bath
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.25 methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O via diiron(IV) reaction intermediate Q, reaction mechanism of the soluble enzyme with different substrates, overview Methylococcus capsulatus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 additional information
-
-
 Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 difluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus difluoromethanol + NAD+ + H2O
-
 ?
1.14.13.25 difluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus Bath difluoromethanol + NAD+ + H2O
-
 ?
1.14.13.25 fluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus fluoromethanol + NAD+ + H2O
-
 ?
1.14.13.25 fluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus Bath fluoromethanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2 via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane Methylococcus capsulatus methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2
-
 Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
 ?
1.14.13.25 methane + NADH + O2 via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
 ?
1.14.13.25 additional information fluoroform is no substrate Methylococcus capsulatus ?
-
 ?
1.14.13.25 additional information fluoroform is no substrate Methylococcus capsulatus Bath ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMO
-
 Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.25 20
-
 assay at Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.25 7
-
 assay at Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus