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Literature summary extracted from

  • Matsuzaki, F.; Wariishi, H.
    Molecular characterization of cytochrome P450 catalyzing hydroxylation of benzoates from the white-rot fungus Phanerochaete chrysosporium (2005), Biochem. Biophys. Res. Commun., 334, 1184-1190.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.92 additional information benzoic acid induces transcription of the gene encoding the enzyme and has a regulatory effect Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.92 gene CYP1f, DNA and amino acid sequence determination and analysis, five introns and six exons, functional expression in Pichia pastoris Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.92 microsome
-
 Phanerodontia chrysosporium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.92 Iron heme iron, the heme-binding domain FSFGPRSCVG at residues 421–430 is located in the C-terminal region Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.92 60000
-
 x * 60000, about, amino acid sequence calculation Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.92 benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 Phanerodontia chrysosporium benzoic acid has a regulatory effect on the enzyme 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.92 Phanerodontia chrysosporium Q4H2B4 white-rot fungus, a lignin-degrading basidiomycete, ATCC 34541
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.92 2-methylbenzoic acid + NADPH + O2
-
 Phanerodontia chrysosporium 4-hydroxy-2-methylbenzoic acid + NADP+ + H2O
-
 ?
1.14.14.92 3-hydroxybenzoic acid + [reduced NADPH-hemoprotein reductase] + O2 reaction of 3-hydroxybenzoate 4-monooxygenase, EC 1.14.13.23 Phanerodontia chrysosporium 3,4-dihydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-methylbenzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Phanerodontia chrysosporium 4-hydroxy-3-methylbenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 benzoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Phanerodontia chrysosporium 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 benzoic acid has a regulatory effect on the enzyme Phanerodontia chrysosporium 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.92 ? x * 60000, about, amino acid sequence calculation Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
1.14.14.92 Cyp1
-
 Phanerodontia chrysosporium
1.14.14.92 More the enzyme belongs to the cytochrome P450 CYP53 family, cf. EC 1.14.13.23 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.92 30
-
 assay at Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.92 7.4
-
 assay at Phanerodontia chrysosporium