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Literature summary extracted from

  • Saxena, R.; Chakraborti, P.K.
    The carboxy-terminal end of the peptide deformylase from Mycobacterium tuberculosis is indispensable for its enzymatic activity (2005), Biochem. Biophys. Res. Commun., 332, 418-425.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 into pET28c vector for over-expression in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.88 C106S inactive mutant Mycobacterium tuberculosis
3.5.1.88 DELTA182-197 deletion mutant, amino acids 182-197 were removed from the carboxy-terminal end, inactive Mycobacterium tuberculosis
3.5.1.88 H148A inactive mutant Mycobacterium tuberculosis
3.5.1.88 H152A inactive mutant Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 1,10-phenanthroline
-
 Mycobacterium tuberculosis
3.5.1.88 actinonin
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 4.1
-
 N-formyl-L-Met-L-Ala
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Fe2+
-
 Mycobacterium tuberculosis
3.5.1.88 Zn2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.88 31000
-
 SDS-PAGE, Western blotting using an anti-his tag antibody Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.88 formyl-L-methionyl peptide + H2O Mycobacterium tuberculosis
-
 formate + methionyl peptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 on Ni-NTA column Mycobacterium tuberculosis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.88 solubilized with 3 M urea Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 formyl-L-methionyl peptide + H2O
-
 Mycobacterium tuberculosis formate + methionyl peptide
-
 ?
3.5.1.88 N-formyl-L-Met-L-Ala + H2O
-
 Mycobacterium tuberculosis formate + L-Met-L-Ala
-
 ?
3.5.1.88 N-formylmethionine-alanine + H2O
-
 Mycobacterium tuberculosis formate + methionyl-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 mPDF
-
 Mycobacterium tuberculosis
3.5.1.88 peptide deformylase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.88 30
-
 mPDF, activity assay Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.88 20 30
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.88 7.4
-
 activity assay Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.88 0.0000197
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 19.7 nM calculated value for the Mycobacterium tuberculosis enzyme Mycobacterium tuberculosis