EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.156 | expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS | Parasynechococcus marenigrum WH 8102 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.156 | 0.3 | - |
S-adenosyl-L-methionine | with glycine as methyl group acceptor, pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
2.1.1.156 | 0.44 | - |
S-adenosyl-L-methionine | with N-methylglycine as methyl group acceptor, pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
2.1.1.156 | 2.94 | - |
N-methylglycine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
2.1.1.156 | 5.22 | - |
glycine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
2.1.1.161 | 0.41 | - |
S-adenosyl-L-methionine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 | |
2.1.1.161 | 2.11 | - |
N,N-dimethylglycine | pH 8.2, 37°C | Parasynechococcus marenigrum WH 8102 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 35000 | - |
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration | Parasynechococcus marenigrum WH 8102 |
2.1.1.156 | 35500 | - |
gel filtration | Parasynechococcus marenigrum WH 8102 |
2.1.1.156 | 38000 | - |
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | 32000 | - |
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | 34000 | - |
gel filtration | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | 36000 | - |
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.156 | S-adenosyl-L-methionine + glycine | Parasynechococcus marenigrum WH 8102 | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | Parasynechococcus marenigrum WH 8102 | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | Parasynechococcus marenigrum WH 8102 | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.156 | Parasynechococcus marenigrum WH 8102 | Q7U4Z8 | - |
- |
2.1.1.161 | Parasynechococcus marenigrum WH 8102 | Q7U4Z9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.156 | recombinant protein using His-tag | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | recombinant protein using His-tag | Parasynechococcus marenigrum WH 8102 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.156 | additional information | several other amino acids tested as substrates and non of them serves as methyl group acceptor | Parasynechococcus marenigrum WH 8102 | ? | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | - |
Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | - |
Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.161 | additional information | several other amino acids tested as substrates and non of them serves as methyl group acceptor | Parasynechococcus marenigrum WH 8102 | ? | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | - |
Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Parasynechococcus marenigrum WH 8102 | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.156 | monomer | 1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | monomer | 1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration | Parasynechococcus marenigrum WH 8102 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.156 | glycine sarcosine N-methyltransferase | - |
Parasynechococcus marenigrum WH 8102 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 8 | - |
with N-methylglycine as substrate | Parasynechococcus marenigrum WH 8102 |
2.1.1.156 | 8.2 | - |
with glycine as substrate | Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | 8.5 | - |
with N,N-dimethylglycin as substrate | Parasynechococcus marenigrum WH 8102 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 7 | 10 | - |
Parasynechococcus marenigrum WH 8102 |
2.1.1.161 | 7 | 10 | - |
Parasynechococcus marenigrum WH 8102 |