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Literature summary extracted from

  • Lu, W.D.; Chi, Z.M.; Su, C.D.
    Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis (2006), Arch. Microbiol., 186, 495-506.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.156 expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS Parasynechococcus marenigrum WH 8102
2.1.1.161 expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS Parasynechococcus marenigrum WH 8102

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.156 0.3
-
S-adenosyl-L-methionine with glycine as methyl group acceptor, pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.1.1.156 0.44
-
S-adenosyl-L-methionine with N-methylglycine as methyl group acceptor, pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.1.1.156 2.94
-
N-methylglycine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.1.1.156 5.22
-
glycine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.1.1.161 0.41
-
S-adenosyl-L-methionine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102
2.1.1.161 2.11
-
N,N-dimethylglycine pH 8.2, 37°C Parasynechococcus marenigrum WH 8102

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.156 35000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102
2.1.1.156 35500
-
gel filtration Parasynechococcus marenigrum WH 8102
2.1.1.156 38000
-
1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102
2.1.1.161 32000
-
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration Parasynechococcus marenigrum WH 8102
2.1.1.161 34000
-
gel filtration Parasynechococcus marenigrum WH 8102
2.1.1.161 36000
-
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration Parasynechococcus marenigrum WH 8102

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.156 S-adenosyl-L-methionine + glycine Parasynechococcus marenigrum WH 8102 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N-methylglycine
-
?
2.1.1.156 S-adenosyl-L-methionine + N-methylglycine Parasynechococcus marenigrum WH 8102 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
2.1.1.161 S-adenosyl-L-methionine + N,N-dimethylglycine Parasynechococcus marenigrum WH 8102 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.156 Parasynechococcus marenigrum WH 8102 Q7U4Z8
-
-
2.1.1.161 Parasynechococcus marenigrum WH 8102 Q7U4Z9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.156 recombinant protein using His-tag Parasynechococcus marenigrum WH 8102
2.1.1.161 recombinant protein using His-tag Parasynechococcus marenigrum WH 8102

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.156 additional information several other amino acids tested as substrates and non of them serves as methyl group acceptor Parasynechococcus marenigrum WH 8102 ?
-
?
2.1.1.156 S-adenosyl-L-methionine + glycine
-
Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N-methylglycine
-
?
2.1.1.156 S-adenosyl-L-methionine + glycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N-methylglycine
-
?
2.1.1.156 S-adenosyl-L-methionine + N-methylglycine
-
Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
2.1.1.156 S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
?
2.1.1.161 additional information several other amino acids tested as substrates and non of them serves as methyl group acceptor Parasynechococcus marenigrum WH 8102 ?
-
?
2.1.1.161 S-adenosyl-L-methionine + N,N-dimethylglycine
-
Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?
2.1.1.161 S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Parasynechococcus marenigrum WH 8102 S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.156 monomer 1 * 38000, SDS-PAGE, 1 * 35000, calculated from the deduced amino acid sequence, 1 * 35500, gel fltration Parasynechococcus marenigrum WH 8102
2.1.1.161 monomer 1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration Parasynechococcus marenigrum WH 8102

Synonyms

EC Number Synonyms Comment Organism
2.1.1.156 glycine sarcosine N-methyltransferase
-
Parasynechococcus marenigrum WH 8102

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.156 8
-
with N-methylglycine as substrate Parasynechococcus marenigrum WH 8102
2.1.1.156 8.2
-
with glycine as substrate Parasynechococcus marenigrum WH 8102
2.1.1.161 8.5
-
with N,N-dimethylglycin as substrate Parasynechococcus marenigrum WH 8102

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.156 7 10
-
Parasynechococcus marenigrum WH 8102
2.1.1.161 7 10
-
Parasynechococcus marenigrum WH 8102