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Literature summary extracted from
- Activity coupling of Vibrio harveyi luciferase and flavin reductase (FRP): oxygen as a probe (2006), Arch. Biochem. Biophys., 454, 26-31.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.3 | expression in Escherichia coli strain JM101 | Vibrio harveyi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.3 | additional information | increases in oxygen concentration lead to gradual decreases of the peak bioluminescence intensity, Km for FMN, and Km for NADPH of NADPH-specific flavin reductase in the coupled reaction with luciferase | Vibrio harveyi |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.30 | additional information | - |
additional information | Km-value of both NADPH and FMN gradually decreases at increasing concentrations of oxygen, with 0.000104 and 0.000019 mM as the upper limits for Km of FMN and NADPH, resp. | Vibrio harveyi | |
| 1.5.1.30 | 0.000019 | - |
NADPH | presence of 1.05 mM O2 | Vibrio harveyi |  |
| 1.5.1.30 | 0.00013 | - |
FMN | presence of 1.05 mM O2 | Vibrio harveyi | |
| 1.14.14.3 | additional information | - |
additional information | kinetics of the FRP:luciferase coupled bioluminescence reaction | Vibrio harveyi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.3 | additional information | Vibrio harveyi | Vibrio harveyi NADPH-specific flavin reductase FRP transfers reduced riboflavin-5'-phosphate to luciferase by both free diffusion and direct transfer, resulting inbioluminescence production, FRP:luciferase coupled bioluminescence reaction, overview, increases in oxygen concentration lead to gradual decreases of the peak bioluminescence intensity, Km for FMN, and Km for NADPH of NADPH-specific flavin reductase in the coupled reaction with luciferase | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.30 | Vibrio harveyi | - |
- |
- |
| 1.14.14.3 | Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.3 | recombinant enzyme from Escherichia coli strain JM101 to over 95% purity | Vibrio harveyi |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.5.1.30 | reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | FRP:luciferase coupled reaction can utilize reduced flavin by both free diffusion and direct transfer | Vibrio harveyi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.30 | FMN + NADPH | reaction may be coupled with luciferase for bioluminescence | Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
| 1.14.14.3 | decanal + FMNH2 + O2 | - |
Vibrio harveyi | decanoate + FMN + H2O + hv | - |
ir | |
| 1.14.14.3 | additional information | Vibrio harveyi NADPH-specific flavin reductase FRP transfers reduced riboflavin-5'-phosphate to luciferase by both free diffusion and direct transfer, resulting inbioluminescence production, FRP:luciferase coupled bioluminescence reaction, overview, increases in oxygen concentration lead to gradual decreases of the peak bioluminescence intensity, Km for FMN, and Km for NADPH of NADPH-specific flavin reductase in the coupled reaction with luciferase | Vibrio harveyi | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.3 | luciferase | - |
Vibrio harveyi |
| 1.14.14.3 | Vibrio harveyi luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.3 | 23 | - |
assay at | Vibrio harveyi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.3 | 0.1 | - |
FMNH2 | purified enzyme | Vibrio harveyi | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.3 | 7 | - |
assay at | Vibrio harveyi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.30 | FMN | - |
Vibrio harveyi | |
| 1.5.1.30 | NADPH | - |
Vibrio harveyi | |
| 1.14.14.3 | FMNH2 | - |
Vibrio harveyi | |
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