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Literature summary extracted from
- Functional characterization of alcohol oxidases from Aspergillus terreus MTCC 6324 (2006), Appl. Microbiol. Biotechnol., 72, 906-911.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.20 | additional information | no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture | Aspergillus terreus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.20 | additional information | no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture | Aspergillus terreus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.7 | 0.03 | - |
benzyl alcohol | pH 8.0, 30°C | Aspergillus terreus |  |
| 1.1.3.13 | 0.005 | - |
n-Heptanol | pH 8.5, 30°C, SCAO | Aspergillus terreus | |
| 1.1.3.13 | 0.028 | - |
n-butanol | pH 8.5, 30°C, SCAO | Aspergillus terreus | |
| 1.1.3.13 | 0.05 | - |
n-Octanol | pH 8.5, 30°C, SCAO | Aspergillus terreus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.3.7 | microsome | - |
Aspergillus terreus | - |
- |
| 1.1.3.13 | microsome | - |
Aspergillus terreus | - |
- |
| 1.1.3.20 | microsome | - |
Aspergillus terreus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.7 | 272000 | - |
native PAGE | Aspergillus terreus |
| 1.1.3.13 | 272000 | - |
above, native PAGE | Aspergillus terreus |
| 1.1.3.20 | 272000 | - |
isozymes H2, H3, and H4, native PAGE | Aspergillus terreus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.7 | benzyl alcohol + O2 | Aspergillus terreus | - |
benzaldehyde + H2O2 | - |
? | |
| 1.1.3.7 | benzyl alcohol + O2 | Aspergillus terreus MTCC 6324 | - |
benzaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | ethanol + O2 | Aspergillus terreus | very low activity in cells grown on n-hexadecane | acetaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | methanol + O2 | Aspergillus terreus | very low activity in cells grown on n-hexadecane | formaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | n-butanol + O2 | Aspergillus terreus | - |
butanaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | n-heptanol + O2 | Aspergillus terreus | - |
heptanaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | n-octanol + O2 | Aspergillus terreus | - |
octanaldehyde + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.7 | Aspergillus terreus | - |
- |
- |
| 1.1.3.7 | Aspergillus terreus MTCC 6324 | - |
- |
- |
| 1.1.3.13 | Aspergillus terreus | - |
- |
- |
| 1.1.3.13 | Aspergillus terreus MTCC 6324 | - |
- |
- |
| 1.1.3.20 | Aspergillus terreus | - |
isolated from the oil-contaminated soil sample, collected from an oil field of Assam, enzyme form H3 | - |
| 1.1.3.20 | Aspergillus terreus MTCC 6324 | - |
isolated from the oil-contaminated soil sample, collected from an oil field of Assam, enzyme form H3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.3.7 | partially by microsome preparation | Aspergillus terreus |
| 1.1.3.13 | native enzyme to homogeneity | Aspergillus terreus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.3.7 | culture condition:n-hexadecane-grown cell | - |
Aspergillus terreus | - |
| 1.1.3.13 | culture condition:n-hexadecane-grown cell | - |
Aspergillus terreus | - |
| 1.1.3.20 | culture condition:n-hexadecane-grown cell | - |
Aspergillus terreus | - |
| 1.1.3.20 | additional information | evaluation of different carbon sources for growth, overview | Aspergillus terreus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.7 | additional information | - |
activity in n-hexadecane-grown cells | Aspergillus terreus |
| 1.1.3.13 | additional information | - |
- |
Aspergillus terreus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.3.13 | 4°C, purified enzyme, 24 h, loss of only 5% of activity | Aspergillus terreus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.7 | benzyl alcohol + O2 | - |
Aspergillus terreus | benzaldehyde + H2O2 | - |
? | |
| 1.1.3.7 | benzyl alcohol + O2 | - |
Aspergillus terreus MTCC 6324 | benzaldehyde + H2O2 | - |
? | |
| 1.1.3.7 | additional information | bifunctional enzyme showing aryl alcohol oxidase activity as well as secondary alcohol oxidase activity, EC 1.1.3.18, substrate specificity, overview | Aspergillus terreus | ? | - |
? | |
| 1.1.3.7 | additional information | bifunctional enzyme showing aryl alcohol oxidase activity as well as secondary alcohol oxidase activity, EC 1.1.3.18, substrate specificity, overview | Aspergillus terreus MTCC 6324 | ? | - |
? | |
| 1.1.3.13 | ethanol + O2 | - |
Aspergillus terreus | acetaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | ethanol + O2 | very low activity in cells grown on n-hexadecane | Aspergillus terreus | acetaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | methanol + O2 | - |
Aspergillus terreus | formaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | methanol + O2 | very low activity in cells grown on n-hexadecane | Aspergillus terreus | formaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | methanol + O2 | - |
Aspergillus terreus MTCC 6324 | formaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | additional information | substrate specificity dependent on growth substrate, overview | Aspergillus terreus | ? | - |
? | |
| 1.1.3.13 | additional information | substrate specificity dependent on growth substrate, overview | Aspergillus terreus MTCC 6324 | ? | - |
? | |
| 1.1.3.13 | n-butanol + O2 | - |
Aspergillus terreus | butanaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | n-heptanol + O2 | - |
Aspergillus terreus | heptanaldehyde + H2O2 | - |
? | |
| 1.1.3.13 | n-octanol + O2 | - |
Aspergillus terreus | octanaldehyde + H2O2 | - |
? | |
| 1.1.3.20 | additional information | the fungal enzyme forms H1, H2, H3, and H4 have similar biochemical properties, but show different substrate specificities from short chain alcohol oxidase for H1, aryl alcohol oxidase for H4, and secondary alcohol oxidase for H2, H3 is a long chain alcohol oxidase, overview | Aspergillus terreus | ? | - |
? | |
| 1.1.3.20 | additional information | the fungal enzyme forms H1, H2, H3, and H4 have similar biochemical properties, but show different substrate specificities from short chain alcohol oxidase for H1, aryl alcohol oxidase for H4, and secondary alcohol oxidase for H2, H3 is a long chain alcohol oxidase, overview | Aspergillus terreus MTCC 6324 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.3.7 | aryl alcohol oxidase | - |
Aspergillus terreus |
| 1.1.3.7 | veratryl alcohol oxidase | - |
Aspergillus terreus |
| 1.1.3.13 | ethanol oxidase | - |
Aspergillus terreus |
| 1.1.3.13 | methanol oxidase | - |
Aspergillus terreus |
| 1.1.3.13 | SCAO | - |
Aspergillus terreus |
| 1.1.3.13 | short chain alcohol oxidase | - |
Aspergillus terreus |
| 1.1.3.20 | fatty alcohol oxidase | - |
Aspergillus terreus |
| 1.1.3.20 | LCAO | - |
Aspergillus terreus |
| 1.1.3.20 | long chain alcohol oxidase | - |
Aspergillus terreus |
| 1.1.3.20 | long chain fatty acid oxidase | - |
Aspergillus terreus |
| 1.1.3.20 | long chain fatty alcohol oxidase | - |
Aspergillus terreus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.7 | 30 | - |
- |
Aspergillus terreus |
| 1.1.3.13 | 30 | - |
- |
Aspergillus terreus |
| 1.1.3.20 | 30 | - |
- |
Aspergillus terreus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.7 | 30 | - |
highly decreased stability beyond 30°C | Aspergillus terreus |
| 1.1.3.13 | 4 | - |
24 h, loss of only 5% of activity | Aspergillus terreus |
| 1.1.3.13 | 30 | - |
the enzyme is highly temperature-sensitive, loss of 20% activity within 100 min | Aspergillus terreus |
| 1.1.3.13 | 35 | - |
30 min, loss of 50% activity, inactivation after 100 min | Aspergillus terreus |
| 1.1.3.13 | 40 | - |
inactivation after 30 min | Aspergillus terreus |
| 1.1.3.20 | 30 | - |
unstable above | Aspergillus terreus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.7 | 8.5 | - |
- |
Aspergillus terreus |
| 1.1.3.13 | 8.5 | - |
- |
Aspergillus terreus |
| 1.1.3.20 | 8.5 | - |
- |
Aspergillus terreus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.13 | 6 | 9 | stable at pH 9.0, loss of 60% activity at pH 6.0, the activity is drastically reduced at below neutral pH and above pH 9.0, overview | Aspergillus terreus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.13 | FAD | bound | Aspergillus terreus | |
| 1.1.3.20 | additional information | no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture | Aspergillus terreus |
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