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Literature summary extracted from

  • Kagle, J.; Hay, A.G.
    Phenylacetylene reversibly inhibits the phenol hydroxylase of Pseudomonas sp. CF600 at high concentrations but is oxidized at lower concentrations (2006), Appl. Microbiol. Biotechnol., 72, 306-315.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.7 ethynylbenzene reversible, competitive inhibition at concentrations above 1 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2 Pseudomonas sp. substrate only for phenol-grown cells 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O
-
 ?
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2 Pseudomonas sp. CF 600 substrate only for phenol-grown cells 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas sp.
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas sp. CF 600
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+ Pseudomonas sp.
-
 phenol + NADP+ + H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+ Pseudomonas sp. CF 600
-
 phenol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2 Pseudomonas sp.
-
 catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2 Pseudomonas sp. CF 600
-
 catechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Pseudomonas sp.
-
-
-
1.14.13.7 Pseudomonas sp. CF 600
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.7 culture condition:phenol-grown cell
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2
-
 Pseudomonas sp. 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O product identification by GC-MS ?
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2 substrate only for phenol-grown cells Pseudomonas sp. 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O
-
 ?
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2
-
 Pseudomonas sp. CF 600 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O product identification by GC-MS ?
1.14.13.7 2 ethynylbenzene + NADPH + 3 O2 substrate only for phenol-grown cells Pseudomonas sp. CF 600 2-ethynylphenol + 2-hydroxy-6-oxo-octa-2,4-dien-7-ynoic acid + NADP+ + H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas sp. 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas sp. CF 600 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+
-
 Pseudomonas sp. phenol + NADP+ + H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+
-
 Pseudomonas sp. CF 600 phenol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas sp. CF 600 catechol + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 phenol hydroxylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 22
-
 assay at room temperature Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.7 7.4
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADPH
-
 Pseudomonas sp.