EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.5 | degradation | degradation of polyethylene glycols, PEGs | Sphingopyxis macrogoltabida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.5 | expression as His-tag fusion protein in Echerichia coli Rosetta (DE3) pLys | Sphingopyxis macrogoltabida |
1.2.1.5 | the pegC gene of Sphingomonas macrogoltabidus is cloned into the pGEM-T and pET23d vectors for expression of nicotinprotein aldehyde dehydrogenase in Escherichia coli Rosetta DE3 pLysS | Sphingopyxis macrogoltabida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | Cd2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | Cu2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | Hg2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | p-chloromercuribenzoate | 50 microM, 80% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | p-mercuribenzoate | 80% inhibition at 0.05 mM | Sphingopyxis macrogoltabida | |
1.2.1.5 | Pb2+ | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | pyrazole | 100% inhibition at 1 mM; 1 mM, 100% inhibition | Sphingopyxis macrogoltabida | |
1.2.1.5 | trans-4-(N,N-dimethylamino)-cinnamaldehyde | 1 mM, 58% inhibition; 58% inhibition at 1 mM | Sphingopyxis macrogoltabida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.5 | 0.137 | - |
n-dodecanal | 50 mM potassium phosphate buffer pH8.0, 1 mM CaCl2, 36°C | Sphingopyxis macrogoltabida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | Ca2+ | - |
Sphingopyxis macrogoltabida | |
1.2.1.5 | Ca2+ | stimulating | Sphingopyxis macrogoltabida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 46100 | - |
SDS-PAGE, recombinant enzyme | Sphingopyxis macrogoltabida |
1.2.1.5 | 46100 | - |
molecular weight of one subunit, determined by gel filtration on a Superdex column and by SDS-PAGE | Sphingopyxis macrogoltabida |
1.2.1.5 | 190000 | - |
190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer | Sphingopyxis macrogoltabida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.5 | an aldehyde + NAD(P)+ + H2O | Sphingopyxis macrogoltabida | - |
an acid + NAD(P)H + H+ | - |
? | |
1.2.1.5 | an aldehyde + NAD(P)+ + H2O | Sphingopyxis macrogoltabida 103 | - |
an acid + NAD(P)H + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.5 | Sphingopyxis macrogoltabida | - |
- |
- |
1.2.1.5 | Sphingopyxis macrogoltabida 103 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.5 | Ni-affinity chromatography | Sphingopyxis macrogoltabida |
1.2.1.5 | on a Ni-NTA column | Sphingopyxis macrogoltabida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.5 | cell culture | - |
Sphingopyxis macrogoltabida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 0.07 | - |
cell free extract | Sphingopyxis macrogoltabida |
1.2.1.5 | 0.49 | - |
after the Ni-NTA purification step | Sphingopyxis macrogoltabida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.5 | acetaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | acetate + NADPH + H+ | - |
? | |
1.2.1.5 | acetaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | acetate + NADPH + H+ | - |
? | |
1.2.1.5 | an aldehyde + NAD(P)+ + H2O | - |
Sphingopyxis macrogoltabida | an acid + NAD(P)H + H+ | - |
? | |
1.2.1.5 | an aldehyde + NAD(P)+ + H2O | - |
Sphingopyxis macrogoltabida 103 | an acid + NAD(P)H + H+ | - |
? | |
1.2.1.5 | formaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | formate + NADPH + H+ | - |
? | |
1.2.1.5 | formaldehyde + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | formate + NADPH + H+ | - |
? | |
1.2.1.5 | n-butanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-butanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-butanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | n-butanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-decanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-decanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-decanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida 103 | n-decanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-dodecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-dodecanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-hexanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-hexanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-nonanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-nonanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-octanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-octanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-pentanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-pentanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-propanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-propanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-tetradecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-tetradecanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-tridecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-tridecanoate + NADPH + H+ | - |
? | |
1.2.1.5 | n-undecanal + NADP+ + H2O | - |
Sphingopyxis macrogoltabida | n-undecanoate + NADPH + H+ | - |
? | |
1.2.1.5 | polyethylene glycol 1000-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
1.2.1.5 | polyethylene glycol 200-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
1.2.1.5 | polyethylene glycol 400-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
1.2.1.5 | polyethylene glycol 4000-aldehyde + NADP+ | highest activtiy compared to other polyethylene glycolaldehydes | Sphingopyxis macrogoltabida | ? + NADPH | - |
? | |
1.2.1.5 | polyethylene glycol 6000-aldehyde + NADP+ | - |
Sphingopyxis macrogoltabida | ? + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.5 | homotetramer | 190000, molecular mass of the native enzyme is estimated by gel filtration, indicating that the enzyme is a homotetramer | Sphingopyxis macrogoltabida |
1.2.1.5 | homotetramer | 4 * 46100, gel filtration | Sphingopyxis macrogoltabida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.5 | aldehyde dehydrogenase | - |
Sphingopyxis macrogoltabida |
1.2.1.5 | ALDH | - |
Sphingopyxis macrogoltabida |
1.2.1.5 | nicotinprotein aldehyde dehydrogenase | - |
Sphingopyxis macrogoltabida |
1.2.1.5 | PEG-ALDH | - |
Sphingopyxis macrogoltabida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 36 | - |
- |
Sphingopyxis macrogoltabida |
1.2.1.5 | 36 | - |
activity assay | Sphingopyxis macrogoltabida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 8 | - |
- |
Sphingopyxis macrogoltabida |
1.2.1.5 | 8 | - |
activity assay | Sphingopyxis macrogoltabida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | NAD+ | - |
Sphingopyxis macrogoltabida | |
1.2.1.5 | NADP+ | - |
Sphingopyxis macrogoltabida | |
1.2.1.5 | NADP+ | 0.94 mol NADP per subunit | Sphingopyxis macrogoltabida |