Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakamoto, T.; Taniguchi, Y.; Suzuki, S.; Ihara, H.; Kawasaki, H.
    Characterization of Fusarium oxysporum beta-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan (2007), Appl. Environ. Microbiol., 73, 3109-3112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.164 gene Fogal1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha as maltose-binding-protein fusion enzyme Fusarium oxysporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.164 Ag+ 1 mM, 50% inhibition Fusarium oxysporum
3.2.1.164 Cd2+ 1 mM, 45% inhibition Fusarium oxysporum
3.2.1.164 Cu2+ 1 mM, 45% inhibition Fusarium oxysporum
3.2.1.164 Fe2+ 1 mM, 65% inhibition Fusarium oxysporum
3.2.1.164 Hg2+ 1 mM, 80% inhibition Fusarium oxysporum
3.2.1.164 additional information no inhibition by EDTA Fusarium oxysporum
3.2.1.164 Zn2+ 1 mM, 60% inhibition Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.164 additional information
-
 additional information
-
 Fusarium oxysporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.164 additional information the enzyme activity does not require metal ions and is not affected by the presence of Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, or Ni2+, overview Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.164 47000
-
 x * 47000, SDS-PAGE Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.164 Fusarium oxysporum
-
 gene Fogal1
-
3.2.1.164 Fusarium oxysporum 12S
-
 gene Fogal1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.164 recombinant maltose-binding-protein fusion enzyme from Escherichia coli strain DH5alpha, by cation exchange and amylose affinity chromatography, followed by hydrophobic interaction chromatography, native enzyme 88fold from strain S12 culture filtrate by anion exchange and hydrophobic interaction chromatography, followed by cation exchange chromatography, dialysis and gel filtration Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.164 1.55
-
 purified native enzyme Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.164 type II arabinogalactans + H2O the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch Fusarium oxysporum ?
-
 ?
3.2.1.164 type II arabinogalactans + H2O the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch Fusarium oxysporum 12S ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.164 ? x * 47000, SDS-PAGE Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.164 beta-1,6-galactanase
-
 Fusarium oxysporum
3.2.1.164 GAL1
-
 Fusarium oxysporum
3.2.1.164 More the enzyme belongs to the glycoside hydrolase family 5 Fusarium oxysporum
3.2.1.164 type II arabinogalactan-degrading enzyme
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.164 50
-
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.164 60
-
 1 h, purified native enzyme, over 80% remaining activity Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.164 5
-
-
 Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.164 4 10 16 h, purified native enzyme, over 80% remaining activity Fusarium oxysporum