EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.9.1 | drug development | the enzyme is a potential herbicide target in C4 plants | Zea mays |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.9.1 | - |
Zea mays |
2.7.9.1 | expressed in Escherichia coli | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.9.1 | ilimaquinone | selectively toxic to C4 plants, isolated from a marine sponge, heterocyclic compound, 3 rings, 2-D structure shown | Zea mays | |
2.7.9.1 | additional information | extracts from several fungal isolates selectively inhibit PPDK; for PPDK inhibition assay 5 and 10 micrograms per ml of unguinol tested in the presence of different substrate concentrations, whole-plant phytotoxicity test (C4, C3),antimicrobial assays and anti-tumor cell assays described for unguinol, 2-D structure shown for further potential candidates to inhibit PPDK that are analogues of unguinol as acarogobien A, acarogobien B and guisinol | Zea mays | |
2.7.9.1 | unguinol | from fungal isolate F3000054, a mixed noncompetitive inhibitor of PPDK with respect to the substrates pyruvate and ATP and an uncompetitive inhibitor of PPDK with respect to phosphate. Unguinol has deleterious effects on a model C4 plant but no effect on a model C3 plant, effects in vivo, overview; mixed noncompetitive inhibition of PPDK with respect to the substrates pyruvate and ATP, uncompetitive with respect to phosphate, phytotoxic effects (bleaching) on C4 plants, no effect on a model C3 plant (Hordeum vulgare) | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.1 | ATP + pyruvate + phosphate | Zea mays | - |
AMP + phosphoenolpyruvate + diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.9.1 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.9.1 | maize PPDK of leaves expressed for inhibitor studies in Escherichia coli with a His-tag and purified on a Ni-affinity column | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.9.1 | leaf | - |
Zea mays | - |
2.7.9.1 | leaf | maize PPDK of leaves used for inhibitor studies | Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.9.1 | ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
2.7.9.1 | ATP + pyruvate + phosphate | different substrate concentrations for PPDK inhibition assay, pyruvate (40, 80, 160, 320, 480, and 720 micromol), ATP (20, 40, 60, 120, 240, and 480 micromol), and phosphate (0.3, 0.6, 1.2, 1.8, 2.7, and 4.32 mM) | Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.9.1 | PPDK | - |
Zea mays |
2.7.9.1 | pyruvate phoshate dikinase | - |
Zea mays |
2.7.9.1 | pyruvate phosphate dikinase | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.9.1 | AMP | reverse reaction | Zea mays | |
2.7.9.1 | ATP | forward reaction | Zea mays |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.9.1 | additional information | - |
additional information | inhibition kinetics for unguinol on PPDK | Zea mays |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.9.1 | 0.04 | - |
- |
Zea mays | unguinol | |
2.7.9.1 | 0.29 | - |
- |
Zea mays | ilimaquinone |