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Literature summary extracted from

  • Motti, C.A.; Bourne, D.G.; Burnell, J.N.; Doyle, J.R.; Haines, D.S.; Liptrot, C.H.; Llewellyn, L.E.; Ludke, S.; Muirhead, A.; Tapiolas, D.M.
    Screening marine fungi for inhibitors of the C4 plant enzyme pyruvate phosphate dikinase: unguinol as a potential novel herbicide candidate (2007), Appl. Environ. Microbiol., 73, 1921-1927.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.9.1 drug development the enzyme is a potential herbicide target in C4 plants Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1
-
Zea mays
2.7.9.1 expressed in Escherichia coli Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 ilimaquinone selectively toxic to C4 plants, isolated from a marine sponge, heterocyclic compound, 3 rings, 2-D structure shown Zea mays
2.7.9.1 additional information extracts from several fungal isolates selectively inhibit PPDK; for PPDK inhibition assay 5 and 10 micrograms per ml of unguinol tested in the presence of different substrate concentrations, whole-plant phytotoxicity test (C4, C3),antimicrobial assays and anti-tumor cell assays described for unguinol, 2-D structure shown for further potential candidates to inhibit PPDK that are analogues of unguinol as acarogobien A, acarogobien B and guisinol Zea mays
2.7.9.1 unguinol from fungal isolate F3000054, a mixed noncompetitive inhibitor of PPDK with respect to the substrates pyruvate and ATP and an uncompetitive inhibitor of PPDK with respect to phosphate. Unguinol has deleterious effects on a model C4 plant but no effect on a model C3 plant, effects in vivo, overview; mixed noncompetitive inhibition of PPDK with respect to the substrates pyruvate and ATP, uncompetitive with respect to phosphate, phytotoxic effects (bleaching) on C4 plants, no effect on a model C3 plant (Hordeum vulgare) Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate Zea mays
-
AMP + phosphoenolpyruvate + diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 maize PPDK of leaves expressed for inhibitor studies in Escherichia coli with a His-tag and purified on a Ni-affinity column Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 leaf
-
Zea mays
-
2.7.9.1 leaf maize PPDK of leaves used for inhibitor studies Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
Zea mays AMP + phosphoenolpyruvate + diphosphate
-
r
2.7.9.1 ATP + pyruvate + phosphate different substrate concentrations for PPDK inhibition assay, pyruvate (40, 80, 160, 320, 480, and 720 micromol), ATP (20, 40, 60, 120, 240, and 480 micromol), and phosphate (0.3, 0.6, 1.2, 1.8, 2.7, and 4.32 mM) Zea mays AMP + phosphoenolpyruvate + diphosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
Zea mays
2.7.9.1 pyruvate phoshate dikinase
-
Zea mays
2.7.9.1 pyruvate phosphate dikinase
-
Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.1 AMP reverse reaction Zea mays
2.7.9.1 ATP forward reaction Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.9.1 additional information
-
additional information inhibition kinetics for unguinol on PPDK Zea mays

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.9.1 0.04
-
-
Zea mays unguinol
2.7.9.1 0.29
-
-
Zea mays ilimaquinone