EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.13.25 | degradation | sMMO can be used for biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene, trans-dichloroethylene, and vinyl chloride. If the concentrations are increased to 0.1 mM, sMMO-expressing cells grow slower and degrade less of these pollutants in a shorter amount of time than pMMO | Methylosinus trichosporium |
1.14.18.3 | degradation | pMMO can be used for biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene, trans-dichloroethylene, and vinyl chloride. If the concentrations are increased to 0.1 mM, pMMO-expressing cells grow faster and degrade more of these pollutants in a shorter amount of time than sMMO | Methylosinus trichosporium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | Acetylene | complete inhibition at 0.1 mM | Methylosinus trichosporium | |
1.14.18.3 | Acetylene | complete inhibition at 0.1 mM | Methylosinus trichosporium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.25 | cytoplasm | - |
Methylosinus trichosporium | 5737 | - |
1.14.13.25 | soluble | - |
Methylosinus trichosporium | - |
- |
1.14.18.3 | membrane | intracytoplasmic membrane | Methylosinus trichosporium | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.25 | additional information | does not require Cu2+ | Methylosinus trichosporium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
1.14.18.3 | Methylosinus trichosporium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.25 | methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + ? | each of these compounds is completely degraded by sMMO-expressing cells when initial concentrations are either 0.01 or 0.03 mM | Methylosinus trichosporium | formaldehyde + ? | - |
? | |
1.14.13.25 | naphthalene + NADH + H+ | - |
Methylosinus trichosporium | alpha-naphthol + beta-naphthol + NAD+ + H2O | - |
? | |
1.14.18.3 | methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + O2 | - |
Methylosinus trichosporium | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.25 | sMMO | - |
Methylosinus trichosporium |
1.14.13.25 | soluble methane monooxygenase | - |
Methylosinus trichosporium |
1.14.18.3 | particulate methane monooxygenase | - |
Methylosinus trichosporium |
1.14.18.3 | pMMO | - |
Methylosinus trichosporium |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.18.3 | Methylosinus trichosporium | for pMMO-expressing conditions, 0.02 mM copper is added and is equilibrated for at least 1 day before the media are inoculated | up |