Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, S.W.; Keeney, D.R.; Lim, D.H.; Dispirito, A.A.; Semrau, J.D.
    Mixed pollutant degradation by Methylosinus trichosporium OB3b expressing either soluble or particulate methane monooxygenase: can the tortoise beat the hare? (2006), Appl. Environ. Microbiol., 72, 7503-7509.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.13.25 degradation sMMO can be used for biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene, trans-dichloroethylene, and vinyl chloride. If the concentrations are increased to 0.1 mM, sMMO-expressing cells grow slower and degrade less of these pollutants in a shorter amount of time than pMMO Methylosinus trichosporium
1.14.18.3 degradation pMMO can be used for biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene, trans-dichloroethylene, and vinyl chloride. If the concentrations are increased to 0.1 mM, pMMO-expressing cells grow faster and degrade more of these pollutants in a shorter amount of time than sMMO Methylosinus trichosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.25 Acetylene complete inhibition at 0.1 mM Methylosinus trichosporium
1.14.18.3 Acetylene complete inhibition at 0.1 mM Methylosinus trichosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 cytoplasm
-
Methylosinus trichosporium 5737
-
1.14.13.25 soluble
-
Methylosinus trichosporium
-
-
1.14.18.3 membrane intracytoplasmic membrane Methylosinus trichosporium 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 additional information does not require Cu2+ Methylosinus trichosporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium
-
-
-
1.14.18.3 Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + ? each of these compounds is completely degraded by sMMO-expressing cells when initial concentrations are either 0.01 or 0.03 mM Methylosinus trichosporium formaldehyde + ?
-
?
1.14.13.25 naphthalene + NADH + H+
-
Methylosinus trichosporium alpha-naphthol + beta-naphthol + NAD+ + H2O
-
?
1.14.18.3 methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + O2
-
Methylosinus trichosporium ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 sMMO
-
Methylosinus trichosporium
1.14.13.25 soluble methane monooxygenase
-
Methylosinus trichosporium
1.14.18.3 particulate methane monooxygenase
-
Methylosinus trichosporium
1.14.18.3 pMMO
-
Methylosinus trichosporium

Expression

EC Number Organism Comment Expression
1.14.18.3 Methylosinus trichosporium for pMMO-expressing conditions, 0.02 mM copper is added and is equilibrated for at least 1 day before the media are inoculated up