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Literature summary extracted from
- Biocatalytic conversion of avermectin to 4"-oxo-avermectin: characterization of biocatalytically active bacterial strains and of cytochrome p450 monooxygenase enzymes and their genes (2005), Appl. Environ. Microbiol., 71, 6968-6976.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.1 | gene family Ema, DNA and amino acid sequence determination and analysis | Streptomyces sp. |
| 1.14.14.1 | gene family ema, DNA and amino acid sequence determination and analysis, overexpression of His-tagged ema1 and ema16 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli and Streptomyces lividans | Streptomyces tubercidicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.1 | additional information | - |
additional information | kinetic analysis of recombinant enzymes | Streptomyces tubercidicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces sp. | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces tubercidicus | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.1 | Streptomyces sp. | - |
several strains, Ema genes | - |
| 1.14.14.1 | Streptomyces tubercidicus | - |
strains R-922 and I-1529, ema genes, and genes cyp229 and cyp230 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.1 | recombinant His-tagged Ema1 and Ema16 from Escherichia coli to near-homogeneity by a two-step purification procedure | Streptomyces tubercidicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.1 | 0.001 | 0.06 | purified recombinant Ema1 and Ema16 proteins | Streptomyces tubercidicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | Streptomyces sp. | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | 4''-oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin, overview | Streptomyces tubercidicus | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | avermectin oxidation activity | Streptomyces sp. | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.1 | avermectin + [reduced NADPH-hemoprotein reductase] + O2 | avermectin oxidation activity | Streptomyces tubercidicus | 4''-oxo-avermectin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.1 | Cyp | - |
Streptomyces sp. |
| 1.14.14.1 | Cyp | - |
Streptomyces tubercidicus |
| 1.14.14.1 | cytochrome P450 monooxygenase | - |
Streptomyces sp. |
| 1.14.14.1 | cytochrome P450 monooxygenase | - |
Streptomyces tubercidicus |
| 1.14.14.1 | Ema | - |
Streptomyces tubercidicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.1 | 30 | - |
assya at | Streptomyces tubercidicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.1 | 0.048 | - |
avermectin | pH 7.0, 30°C, recombinant Ema1 | Streptomyces tubercidicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.1 | 7 | - |
assay at | Streptomyces tubercidicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.1 | Ferredoxin | - |
Streptomyces sp. | |
| 1.14.14.1 | Ferredoxin | - |
Streptomyces tubercidicus |
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