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Literature summary extracted from

  • Cafaro, V.; Notomista, E.; Capasso, P.; Di Donato, A.
    Regiospecificity of two multicomponent monooxygenases from Pseudomonas stutzeri OX1: molecular basis for catabolic adaptation of this microorganism to methylated aromatic compounds (2005), Appl. Environ. Microbiol., 71, 4736-4743.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 the enzyme is encoded in the ph operon, expression in Escherichia coli strain JM109 Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.7 additional information
-
 additional information kinetics and regioselectivity, overview Pseudomonas stutzeri
1.14.13.7 0.0006
-
 phenol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.0006
-
 4-Cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.0018
-
 3-cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.0031
-
 2-Cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.022
-
 Toluene 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.025
-
 Benzene 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.0325
-
 2-xylene 25°C, recombinant enzyme Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Pseudomonas stutzeri
-
-
-
1.14.13.7 Pseudomonas stutzeri OX1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 2 2-cresol + NADPH + O2
-
 Pseudomonas stutzeri 3-methylcatechol + 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri OX1 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 regioselectivity, overview Pseudomonas stutzeri 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3 toluene + 3 NADPH + 3 H+ + 3 O2 regioselectivity, overview Pseudomonas stutzeri OX1 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
1.14.13.7 3-cresol + NADPH + O2
-
 Pseudomonas stutzeri 3-methylcatechol + 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 4-cresol + NADPH + O2 best substrate Pseudomonas stutzeri 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+
-
 Pseudomonas stutzeri phenol + NADP+ + H2O
-
 ?
1.14.13.7 benzene + NADPH + O2 + H+
-
 Pseudomonas stutzeri OX1 phenol + NADP+ + H2O
-
 ?
1.14.13.7 additional information the enzyme hydroxylates benzenes to catechols via the intermediate production of phenols Pseudomonas stutzeri ?
-
 ?
1.14.13.7 additional information the enzyme hydroxylates benzenes to catechols via the intermediate production of phenols Pseudomonas stutzeri OX1 ?
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas stutzeri catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + O2
-
 Pseudomonas stutzeri OX1 catechol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.7 More the enzyme is a multicomponent monooxygenase Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 phenol hydroxylase
-
 Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 25
-
 assay at Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.7 0.1
-
 Benzene 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.125
-
 2-xylene 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.15
-
 Toluene 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.61
-
 3-cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.77
-
 4-Cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 0.95
-
 2-Cresol 25°C, recombinant enzyme Pseudomonas stutzeri
1.14.13.7 1.02
-
 phenol 25°C, recombinant enzyme Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADPH
-
 Pseudomonas stutzeri