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Literature summary extracted from

  • Usuda, Y.; Kurahashi, O.
    Effects of deregulation of methionine biosynthesis on methionine excretion in Escherichia coli (2005), Appl. Environ. Microbiol., 71, 3228-3234.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.46 biotechnology enzyme methionine biosynthesis deregulation mutant strains are useful for construction/production of L-methionine excreting strains Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.46 gene metA, expressionof wild-type and mutant enzymes in strain JM109 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.46 I296S point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A4 Escherichia coli
2.3.1.46 additional information construction of IS-insertion mutant metA7, with 298Pro-Tyr-Asp-Leu-Arg-His-Met-Asn-Pro-Thr-Leu-Asp-stop to 298Arg-Leu-Ala-Pro-stop, obtained from norleucine-resistant strain, and IS insertion mutant metA8, with 298Pro-Tyr-Asp-Leu-Arg-His-Met-Asn-Pro-Thr-Leu-Asp-stop to 298Arg-Leu-Ala-Pro-stop, also obtained from norleucine-resistant strain, overview, introduction of deletion mutations of metJ and thrBC into the W3110 strain has a significant effect on the amount of Met excreted into the medium, overview Escherichia coli
2.3.1.46 P298L point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A5 Escherichia coli
2.3.1.46 R27C point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A9 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.46 L-methionine feedback inhibition Escherichia coli
2.3.1.46 S-adenosyl-L-methionine feedback inhibition Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.46 additional information Escherichia coli the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview ?
-
?
2.3.1.46 additional information Escherichia coli W3110 / ATCC 27325 the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview ?
-
?
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli a step in biosynthesis and regulation of methionine, overview CoA + O-succinyl-L-homoserine
-
?
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli W3110 / ATCC 27325 a step in biosynthesis and regulation of methionine, overview CoA + O-succinyl-L-homoserine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.46 Escherichia coli
-
gene metA
-
2.3.1.46 Escherichia coli W3110 / ATCC 27325
-
gene metA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.46 additional information the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview Escherichia coli ?
-
?
2.3.1.46 additional information the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview Escherichia coli W3110 / ATCC 27325 ?
-
?
2.3.1.46 succinyl-CoA + L-homoserine
-
Escherichia coli CoA + O-succinyl-L-homoserine
-
?
2.3.1.46 succinyl-CoA + L-homoserine a step in biosynthesis and regulation of methionine, overview Escherichia coli CoA + O-succinyl-L-homoserine
-
?
2.3.1.46 succinyl-CoA + L-homoserine
-
Escherichia coli W3110 / ATCC 27325 CoA + O-succinyl-L-homoserine
-
?
2.3.1.46 succinyl-CoA + L-homoserine a step in biosynthesis and regulation of methionine, overview Escherichia coli W3110 / ATCC 27325 CoA + O-succinyl-L-homoserine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.46 homoserine succinyltransferase
-
Escherichia coli
2.3.1.46 MetA
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.46 30
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.46 7.5
-
assay at Escherichia coli