EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.46 | biotechnology | enzyme methionine biosynthesis deregulation mutant strains are useful for construction/production of L-methionine excreting strains | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.46 | gene metA, expressionof wild-type and mutant enzymes in strain JM109 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.46 | I296S | point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A4 | Escherichia coli |
2.3.1.46 | additional information | construction of IS-insertion mutant metA7, with 298Pro-Tyr-Asp-Leu-Arg-His-Met-Asn-Pro-Thr-Leu-Asp-stop to 298Arg-Leu-Ala-Pro-stop, obtained from norleucine-resistant strain, and IS insertion mutant metA8, with 298Pro-Tyr-Asp-Leu-Arg-His-Met-Asn-Pro-Thr-Leu-Asp-stop to 298Arg-Leu-Ala-Pro-stop, also obtained from norleucine-resistant strain, overview, introduction of deletion mutations of metJ and thrBC into the W3110 strain has a significant effect on the amount of Met excreted into the medium, overview | Escherichia coli |
2.3.1.46 | P298L | point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A5 | Escherichia coli |
2.3.1.46 | R27C | point mutation in gene metA leading to a deregulated methionine biosynthesis in the mutant strain A9 | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.46 | L-methionine | feedback inhibition | Escherichia coli | |
2.3.1.46 | S-adenosyl-L-methionine | feedback inhibition | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.46 | additional information | Escherichia coli | the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview | ? | - |
? | |
2.3.1.46 | additional information | Escherichia coli W3110 / ATCC 27325 | the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview | ? | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | Escherichia coli | a step in biosynthesis and regulation of methionine, overview | CoA + O-succinyl-L-homoserine | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | Escherichia coli W3110 / ATCC 27325 | a step in biosynthesis and regulation of methionine, overview | CoA + O-succinyl-L-homoserine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.46 | Escherichia coli | - |
gene metA | - |
2.3.1.46 | Escherichia coli W3110 / ATCC 27325 | - |
gene metA | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.46 | additional information | the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview | Escherichia coli | ? | - |
? | |
2.3.1.46 | additional information | the enzyme is important in regulation of Met biosynthesis and metabolism, metA expression is regulated by the methionine repressor metJ, MetJ and MetA enzyme mutations lead to pathway deregulation and excess methionine production and excretion from the cell, overview | Escherichia coli W3110 / ATCC 27325 | ? | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | - |
Escherichia coli | CoA + O-succinyl-L-homoserine | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | a step in biosynthesis and regulation of methionine, overview | Escherichia coli | CoA + O-succinyl-L-homoserine | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | - |
Escherichia coli W3110 / ATCC 27325 | CoA + O-succinyl-L-homoserine | - |
? | |
2.3.1.46 | succinyl-CoA + L-homoserine | a step in biosynthesis and regulation of methionine, overview | Escherichia coli W3110 / ATCC 27325 | CoA + O-succinyl-L-homoserine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.46 | homoserine succinyltransferase | - |
Escherichia coli |
2.3.1.46 | MetA | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.46 | 30 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.46 | 7.5 | - |
assay at | Escherichia coli |