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Literature summary extracted from

  • Quaratino, D.; Federici, F.; Petruccioli, M.; Fenice, M.; D'Annibale, A.
    Production, purification and partial characterisation of a novel laccase from the white-rot fungus Panus tigrinus CBS 577.79 (2007), Antonie van Leeuwenhoek, 91, 57-69.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Lentinus tigrinus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ presence of type-1 site and type-3 binuclear copper in the bright blue colored purified enzyme Lentinus tigrinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 68500
-
 1 * 68500, SDS-PAGE Lentinus tigrinus
1.10.3.2 69100
-
 gel filtration Lentinus tigrinus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Lentinus tigrinus
-
-
-
1.10.3.2 Lentinus tigrinus CBS 577.79
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 6.9% N-glycosylation of the high mannose type, lectin-binding assays Lentinus tigrinus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 16.7fold from strain 577.79 by ultrafiltration, anion exchange chromatography, and gel filtration to homogeneity Lentinus tigrinus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information produced in a bubble column reactor using glucose-containing medium supplemented with 2,5-xylidine under conditions of nitrogen sufficiency Lentinus tigrinus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 1.71
-
 crude extracellular enzyme in culture Lentinus tigrinus
1.10.3.2 317
-
 purified enzyme Lentinus tigrinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 1-hydroxybenzotriazole + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 1-hydroxybenzotriazole + O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.10.3.2 2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Lentinus tigrinus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. cerulignone ?
1.10.3.2 2-methoxyphenol + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 4-methoxyphenol + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 4-methyl catechol + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 catechol + O2
-
 Lentinus tigrinus ?
-
 ?
1.10.3.2 catechol + O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.10.3.2 additional information substrate specificity, overview Lentinus tigrinus ?
-
 ?
1.10.3.2 additional information substrate specificity, overview Lentinus tigrinus CBS 577.79 ?
-
 ?
1.10.3.2 violuric acid + O2
-
 Lentinus tigrinus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 68500, SDS-PAGE Lentinus tigrinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 55
-
-
 Lentinus tigrinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 55 temperature-activity profile at pH 3.8 Lentinus tigrinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 purified enzyme, half-life: 281 min Lentinus tigrinus
1.10.3.2 60
-
 purified enzyme, half-life: 25 min Lentinus tigrinus
1.10.3.2 70
-
 purified enzyme, half-life: 4 min Lentinus tigrinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 2.6
-
 1-hydroxybenzotriazole pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 7.8
-
 2,2,6,6-tetramethylpiperidin-N-oxide radical pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 8.4
-
 violuric acid pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 17.7
-
 2,6-dimethoxyphenol pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 22.8
-
 2-methoxyphenol pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 34.4
-
 catechol pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 36.3
-
 4-methoxyphenol pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 93.9
-
 4-methyl catechol pH 3.8, 55°C Lentinus tigrinus
1.10.3.2 365.6
-
 2,6-dimethoxyphenol pH 3.8, 55°C Lentinus tigrinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.7 3.8
-
 Lentinus tigrinus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2.5 6.5 3% of maximal activity at pH 6.5, pH-activity profile at 30°C Lentinus tigrinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 additional information
-
-
 Lentinus tigrinus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Lentinus tigrinus isoelectric focusing
-
 3.15