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Literature summary extracted from

  • Brillouet, J.M.; Williams, P.; Moutounet, M.
    Purification and some properties of a novel endo-beta-(1-6)-D-galactanase from Aspergillus niger (1991), Agric. Biol. Chem., 55, 1565-1571.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.164 additional information
-
 additional information
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.164 29000
-
 gel filtration Aspergillus niger
3.2.1.164 60000
-
 native PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.164 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.164 native enzyme 26.2fold to homogeneity by several chromatographic steps, overview Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.164 commercial preparation crude enzyme extract Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.164 25.29
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.164 desarabinosylated grape 3,6-galactan-protein + H2O the enzyme liberates beta-1,6-D-galactobiose and galactose Aspergillus niger ?
-
 ?
3.2.1.164 additional information the enzyme does not cleave beta-D-1,3-galactosidic bonds Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.164 monomer 1 * 60000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.164 endo-beta-(1-6)-D-galactanase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.164 60
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.164 20 75 activity range Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.164 30
-
 4 h, purified enzyme, 1 M acetate buffer, pH 4.2, completely stable Aspergillus niger
3.2.1.164 45
-
 4 h, purified enzyme, 1 M acetate buffer, pH 4.2, 50% remaining activity Aspergillus niger
3.2.1.164 60
-
 4 h, purified enzyme, 1 M acetate buffer, pH 4.2, 17% remaining activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.164 3.5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.164 2 7.5 activity range, 85% of maximal activity at pH 2.5 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.164 2 7.5 2 h, 40°C, 50% remaining activity at pH 2.0 and at pH 7.5 Aspergillus niger