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Literature summary extracted from

  • Tripathi, S.M.; Ramachandran, R.
    Overexpression, purification and crystallization of lysine epsilon-aminotransferase (Rv3290c) from Mycobacterium tuberculosis H37Rv (2006), Acta Crystallogr. Sect. F, F62, 572-575.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.36 hanging drop vapour diffusion method in 0.1 M trisodium citrate dihydrate solution containing 0.2 M ammonium acetate and 25% PEG 4000 in the pH range 5.4-6.0 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.36 50000
-
 SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.36 Mycobacterium tuberculosis
-
-
-
2.6.1.36 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.36 Ni2+-IDA column chromatography and Superdex S-200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.36 L-lysine + 2-oxoglutarate
-
 Mycobacterium tuberculosis 2-aminoadipate 6-semialdehyde + L-glutamate
-
 r
2.6.1.36 L-lysine + 2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv 2-aminoadipate 6-semialdehyde + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.36 dimer gel filtration Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.36 LAT
-
 Mycobacterium tuberculosis
2.6.1.36 lysine epsilon-aminotransferase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.36 pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis