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Literature summary extracted from

  • Egerer-Sieber, C.; Herl, V.; Müller-Uri, F.; Kreis, W.; Muller, Y.A.
    Crystallization and preliminary crystallographic analysis of selenomethionine-labelled progesterone 5beta-reductase from Digitalis lanata Ehrh (2006), Acta Crystallogr. Sect. F, 62, 186-188.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.1.3 additional information inspection of the MAD-phased electron-density map shows that 5beta-POR is a Rossmann-type reductase and the quality of the map is such that it is anticipated that a complete atomic model of 5beta-POR may readily be built Digitalis lanata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 overproduced by the pQE expression system and transformed into Escherichia coli strain B834(DE3) Digitalis lanata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.3 by the hanging-drop method, at 2.7 A resolution, crystals belong to space group P43212, contain a single 5beta-POR molecule in the asymmetric unit and tend to diffract anisotropically, identification of six out of eight possible Se-atom positions Digitalis lanata

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Digitalis lanata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 to homogeneity by Ni–NTA affinity chromatography Digitalis lanata

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 5beta-POR
-
 Digitalis lanata
1.3.1.3 progesterone 5beta-reductase
-
 Digitalis lanata