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Literature summary extracted from
- Structure of the putative mutarotase YeaD from Salmonella typhimurium: structural comparison with galactose mutarotases (2007), Acta Crystallogr. Sect. D, 63, 197-205.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.3 | cloned into pRSET-C vector (Invitrogen), expression in Escherichia coli | Salmonella enterica |
| 5.1.3.3 | gene yeaD, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | overexpression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.1.3.3 | hanging-drop vapour-diffusion method | Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | purified recombinant His-tagged enzyme, hanging-drop vapour-diffusion method, 0.003 ml of 10 mg/ml protein in 15-25% PEG 8000, 0.1 M Na citrate, pH 5.5, and 0.2-0.5 mM NaCl for monoclinic crystals, or in 1.5-2 M ammonium sulfate, 25 mM Mg formate, 0.1 M Na cacodylate, pH 5.5, for orthorhombic crystals, cocrystallization of YeaD with ligands glucose, galactose, mannose, fructose, fucose, xylose, lyxose, lactose, maltose, sucrose and glucose 6-phosphate, incubation at 4°C for 12 h in a 1:10 molar ratio, X-ray diffraction structure determination and analysis the orthorhombic form at 1.9 A resolution and in the monoclinic form at 2.5 A resolution | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 34000 | - |
SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | 34000 | - |
recombinant His-tagged enzyme, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.3 | Salmonella enterica | - |
- |
- |
| 5.1.3.3 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| 5.1.3.3 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene yeaD | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.3 | NiNTA affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | NiNTA affinity column chromatography | Salmonella enterica |
| 5.1.3.3 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.3 | additional information | identification of residues involved in catalysis and substrate binding, role of Arg74 and Arg99, found only in YeaD-like proteins, in ligand anchoring, the specificity of YeaD is different from those of galactose mutarotase, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.3 | monomer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | monomer | 1 x 34000, recombinant His-tagged enzyme, SDS-PAGE, the enzyme is a monomer in solution, but two monomers A and B related by a noncrystallographic twofold axis are found in the asymmetric unit of the orthorhombic form, while two pairs of such monomers (AB and CD) are observed in the monoclinic crystal form, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | More | YeaD adopts the beta-sandwich fold, sugar ligand binding structure, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.3 | aldose 1-epimerase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | aldose 1-epimerase | - |
Salmonella enterica |
| 5.1.3.3 | Mutarotase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | mutarotase YeaD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | mutarotase YeaD | - |
Salmonella enterica |
| 5.1.3.3 | stYeaD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 5.1.3.3 | YeaD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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