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Literature summary extracted from

  • Lim, J.S.; Park, S.W.; Kim, S.W.
    Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization (2006), World J. Microbiol. Biotechnol., 22, 39-44.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.93 synthesis production of 7-aminocephalosporanate on a large scale at 25°C using enzyme immobilized by epoxide silanization Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.5.1.93 the enzyme is thermostabilized by immobilization using epoxide silanization Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Pseudomonas sp.
-
 recombinant
-
3.5.1.93 Pseudomonas sp. KAC-1
-
 recombinant
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. KAC-1 7-aminocephalosporanate + glutarate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 GL-7-ACA acylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 50
-
 soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.93 30 55 30°C: soluble enzyme shows about 45% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 75% of maximal activity, 55°C: soluble enzyme shows about 40% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 55% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.93 37
-
 60 min, soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization lose about 10% of its activity Pseudomonas sp.
3.5.1.93 50
-
 60 min, soluble enzyme loses 77% of its activity, enzyme immobilized on silica gel modified by epoxide silanization loses 60% of its activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 8 8.5 soluble enzyme and enzyme immobilized on silica gel modified by epoxide silanization Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.93 6 10 pH 6.0: soluble enzyme shows about 35% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 65% of maximal activity, pH 10.0: soluble enzyme shows about 70% of maximal activity, enzyme immobilized on silica gel modified by epoxide silanization shows about 85% of maximal activity Pseudomonas sp.